Underphosphorylated Retinoblastoma Mouse anti-Human, Unlabeled, Clone: G99-549, BD

Description

• The retinoblastoma gene encodes a nuclear phosphoprotein (Rb or p110Rb) which is expressed in most normal cells of vertebrates and acts as a tumor suppressor gene product
• An underphosphorylated form of Rb is mainly found in resting or fully differentiated cells, whereas the hyperphosphorylated form is present in proliferating cells
• Only the underphosphorylated form of Rb binds specifically to viral oncogenes such as SV40 large T, adenoviral EIA and HPV-E7
• This interaction may partially contribute to the transforming activity of these viral oncoproteins
• Rb also interacts with several cyclins including A, D, and E as well as the transcriptional activator E2F
• The importance of these interactions for the biological function of Rb is still being elucidated
• G99-549 recognizes only the fast migrating, underphosphorylated form of Rb protein
• G99-549 cross-reacts with mouse underphosphorylated Rb
• The epitope has been mapped to amino acids 514-610 (box A) of human Rb
• Recombinant full-length human Rb was produced in the baculovirus expression vector system (BEVS), purified and used as immunogen
• Host Species: Mouse Clone: G99-549 Isotype: IgG1 Species Reactivity: Human Immunogen: Recombinant full-length human Rb Formula Weight [Chemical]: 105kDa Immunoprecipitation, Western Blotting