Calcineurin Mouse anti-Human, Unlabeled, Clone: G182-1847, BD

Description

• Calcineurin is a Ca2+- and calmodulin-dependent serine/threonine phosphatase which consists of two subunits
• The A subunit (∽61 kDa) is catalytic and has calmodulinbinding activity; the B subunit (∽19 kDa), which is myristylated at its N-terminus, has Ca[2+]-binding activity
• Calcineurin is expressed ubiquitously in eukaryotic cells, including yeast
• The protein is highly expressed in mammalian brain, and has been implicated in a number of calcium-dependent pathways in the nervous system, including regulation of neurotransmitter release
• Calcineurin also plays a key role in T cell receptor-mediated signal transduction pathways
• Calcineurin dephosphorylates components of NF-AT (Nuclear Factor of Activated T cells), allowing NF-AT to translocate to the nucleus where it participates with AP-1 in activation of multiple cytokine and surface receptor genes
• Inhibition of calcineurin by immunosuppressive agents such as cyclosporin A and FK506 correlates with the suppression of early T cell activation events
• These immunosuppressive agents bind to specific intracellular proteins, called immunophilins, to form complexes that inhibit calcineurin’s phosphatase activity
• In the presence of these complexes, NF-AT remains phosphorylated and thus cannot translocate to the nucleus
• A novel protein, Cain (calcineurin inhibitor), has been described which displays a similar pattern of neuronal and brain expression and has been shown to bind and inhibit calcineurin activity in vivo
• G182-1847 recognizes the α and β isoforms of the A subunit of calcineurin (∼61 kDa)
• It reacts with an epitope in the autoinhibitory domain
• It does not recognize the B subunit of calcineurin
• A recombinant tag-fusion protein containing amino acids 349-505 of the human β1 isoform of calcineurin A subunit was used as immunogen.