Bcl-2 w/control Mouse anti-Human, FITC, Clone: BCL-2/100, BD

Description

• Programmed cell death (apoptosis) is a normal physiologic process which occurs during embryonic development as well as in maintenance of tissue homeostasis
• The apoptotic program is characterized by certain morphological features
• These include changes in the plasma membrane such as loss of membrane asymmetry and attachment, a condensation of the cytoplasm and nucleus, and internucleosomal cleavage of DNA
• In the final stages, the dying cells become fragmented into “apoptotic bodies” which are rapidly eliminated by phagocytic cells without eliciting significant inflammatory damage to surrounding cells
• Members of the Bcl-2 family play a major role in regulating the response of cells to apoptotic signals
• Bcl-2 is considered to be novel among proto-oncogenes because it blocks apoptosis in many cell types
• Bcl-2 is thought to provide selective survival advantage for cells by blocking apoptosis and thus may contribute to tumorigenesis
• Bcl-2 is a 26 kDa intracellular, integral membrane protein found primarily in the nuclear envelope, endoplasmic reticulum and outer mitochondrial membrane
• Clone Bcl-2/100 reacts with human Bcl-2
• It does not cross-react with mouse Bcl-2
• A synthetic peptide corresponding to amino acids 41-54 (GAAPAPGIFSSQPG) of human Bcl-2 was used as immunogen
• This peptide sequence is not conserved between human and mouse
• Clone Bcl-2/100 was initially characterized by western blotting and immunohistochemistry of frozen and paraffin-embedded tissue sections
• Clone MOPC-21 is a mouse IgG1 isotype control
• The MOPC-21 antibody has unknown specificity
• The Bcl-2/100 and MOPC-21 FITC conjugates are matched in F/P ratios
• The optimum F/P ratio was experimentally determined by flow cytometric analysis.