MDM2 Mouse, Unlabeled, Clone: SMP14, BD

Description

• MDM2, originally described as a gene product of mouse double minute chromosomes, is a nuclear oncoprotein that can inhibit the action of certain tumor suppressor proteins
• For example, MDM2 binds to the acidic activation domain (residues 2042) of the p53 tumor suppressor protein, and the p53-MDM2 complex down regulates the transcriptional activity of p53
• p53 plays a role in the normal cell cycle by activating the transcription of genes that cause arrest in G1
• The expression of MDM2 is itself, induced by p53 and may be a way for p53 to self-regulate its activity during the normal cell cycle
• However, overexpression of MDM2 results in the loss of p53-regulated growth control and consequently, deregulated cell proliferation
• MDM2 also binds to the Retinoblastoma tumor suppressor protein (Rb) and inhibits its growth regulatory function
• MDM2 can directly augment proliferation by binding to two transcription factors E2F1 and DP1, and stimulating the activity of the S-phase inducing E2F1/DP1 heterodimer
• MDM2 migrates at a reduced molecular weight of ∽95kDa
• The SMP14 clone has been reported to recognize human, mouse and rat MDM2 while exhibiting a slight cross-reactivity with cytokeratins 6, 14 and 16 in some experimental systems
• In the immunoprecipitation application, SMP14 has been reported to precipitate MDM2 and p53-MDM2 complexes
• MCF7 human breast carcinoma cells (ATCC HTB-22) and NIH/3T3 mouse fibroblasts (ATCC CRL-1658) are suggested as western blot and immunoprecipitation positive controls
• SMP14 has been reported to be useful for the immunohistochemical staining of acetone-fixed, frozen sections and of formalin-fixed, paraffin-embedded tissue sections
• In addition to a nuclear staining of MDM2, cytoplasmic staining may also be observed which is likely to be atrributable to the slight crossreactivity of the SMP14 clone with cytokeratins
• This antibody is routinely tested by Western blot analysis, immunofluorescent staining and immunoprecipiation
• Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature
• Host Species: Mouse Clone: SMP14 Isotype: IgG1 Species Reactivity: Human Immunogen: Human MDM2 aa
• 154-167 Peptide Bioimaging, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blotting