AF6 Mouse, Unlabeled, Clone: 35, BD

Description

• AF6 (ALL-1 Fusion partner in chromosome 6) was discovered as the fusion partner of ALL-1
• These genes undergo translocation and fusion in acute myeloid leukemias
• AF6, or p180, was also identified as a Ras-interacting protein isolated from bovine brain
• AF6 binds to the GTP-Ras but not to the inactive GDP, or ineffective forms of Ras
• The interaction between AF6 and Ras may regulate the Ras signaling pathway since this association was prevented by the binding of c-Raf-1 to Ras
• The Ras-binding site is located within amino acids 36-206 of AF6
• AF6 is 1612 amino acids in length with homology to the DLG family of proteins located in cell-cell junctions, suggesting AF6 might be important in cell-to-cell communicative events
• Furthermore, AF6 has the GLGF motif characteristic of DLG proteins that include Drosophilia's Canoe, implicated in the developmental pathway triggered by Notch activation
• Like AF6, Canoe was also identified as a Ras-binding protein
• The amino acid sequence and biochemical property similarities between Canoe and AF6 indicate that the latter may be important in developmental processes regulated by Ras
• Immunofluorescence, Immunoprecipitation, Western Blotting