Carboxypeptidase E Mouse, Unlabeled, Clone: 35, BD

Description

• Carboxypeptidase E (CPE), also known as carboxypeptidase H and enkephalin convertase, is found as both a membrane-bound and a soluble glycoprotein in neuroendocrine tissues and adrenal-gland chromaffin granules
• The C-terminus forms an amphiphilic α-helix, suggesting that this region is responsible for the membrane-bound form
• Evidence suggests the active form of CPE is located in the secretory vesicles
• CPE appears to have several functions
• It is an exopeptidase that cleaves neuropeptides with C-terminal basic amino acids, producing an active form of the peptide
• It has also been proposed that membrane-bound CPE is a sorting receptor for regulated secretory pathway (RSP) proteins in the TGN pituitary Golgi and secretory granule membranes
• RSP proteins primarily consist of hormones and neuropeptides
• Mice that carry a mutation in the CPE gene Cpe[fat] display endocrine disorders such as obesity, infertility, and hyperproinsulinemia
• Furthermore, the same endocrine disorders are observed in Cpe[fat] mice where the CPE gene has been effaced by antisense RNA
• Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting