IAK1 Mouse, Unlabeled, Clone: 4, BD

Description

• Cell division is a tightly regulated process that ensures the segregation of chromosomes into daughter cells
• Essential to this regulation is the modification of cell cycle components by reversible phosphorylation
• Ipl1 and aurora are two related kinases isolated from S.cerevisiae and Drosophila, respectively
• Inactivation of these kinases results in abnormal chromosome segregation and disruption of the centrosome
• A structurally and functionally similar kinase, IAK1 (Ipl1- and Aurora-related kinase 1), is a regulator of mammalian chromosome segregation
• Although IAK1 may be present in the cytoplasm, it is detected on the centrosome following duplication and also associates with the spindle microtubules from metaphase through cell division
• Expression of IAK1 is stringently regulated during the cell cycle
• Both mRNA and protein are initially expressed in S-phase, are elevated during M-phase, and are undetectable following completion of mitosis
• Increasing evidence suggests that IAK1 belongs to a novel subfamily of the ser/thr kinase superfamily
• Although mutational analysis of IAK1 will directly determine its function, it appears to be a key player in the control of cell division
• Immunofluorescence, Western Blotting