CD23 Rat anti-Mouse, BUV395, Clone: B3B4, BD Optibuild™

Description

• The B3B4 monoclonal antibody specifically binds to CD23, the low affinity IgE Fc receptor (Fc ε RII) expressed on mature resting conventional B lymphocytes, but not on B-1 cells (CD5+ B cells) or T lymphocytes
• It does not react with high-affinity IgE receptors, as demonstrated on mouse mast cell lines
• The regulation of CD23 surface expression on activated B cells appears to be complex, depending upon the mode of activation and the presence of cytokines
• IgE synthesis is negatively regulated by CD23, and CD23 expression is upregulated on splenocytes in the presence of IgE
• CD23 is also upregulated on follicular dendritic cells in the lymph nodes of immunized mice, and a subset of splenic dendritic cells expresses CD23
• The B3B4 antibody abrogates antigen-specific IgE-dependent modulation of immune responses in normal mice
• This monoclonal antibody also blocks IgE binding and eosinophil infiltration in the lung of immunized mice
• Different in vivo results have been obtained when using the intact B3B4 antibody or the F(ab')2 fragments
• B3B4 mAb does not cross-react with rat or human IgE Fc Receptor
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348nm and an Em Max at 395nm, BD Horizon BUV395 can be excited with a 355nm laser and detected with a 379/28 filter.