CD23 Mouse anti-Human, Brilliant Violet 605, Clone: M-L233, BD Optibuild™

Description

• The M-L233 antibody specifically binds to human CD23, the low affinity receptor for human IgE (Fc ε RII)
• CD23 is a type II membrane glycoprotein that can be expressed by B cells, monocytes, macrophages, eosinophils, platelets and dendritic cells
• CD23 can mediate IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils
• Soluble CD23 (sCD23) can be released by CD23-positive cells as a result of proteolytic cleavage of membrane CD23
• Larger fragments of sCD23 (e.g., 25-37 kDa) retain their IgE-binding capacity whereas smaller fragments (i.e., ≤ 12 kDa) do not
• Soluble CD23 may have immunoregulatory effects on the growth and differentiation of B cells and other cell types
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.