CD23 Mouse anti-Human, Brilliant Violet 650, Clone: M-L233, BD Optibuild™

Description

• The M-L233 antibody specifically binds to human CD23, the low affinity receptor for human IgE (Fc ε RII)
• CD23 is a type II membrane glycoprotein that can be expressed by B cells, monocytes, macrophages, eosinophils, platelets and dendritic cells
• CD23 can mediate IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils
• Soluble CD23 (sCD23) can be released by CD23-positive cells as a result of proteolytic cleavage of membrane CD23
• Larger fragments of sCD23 (e.g., 25-37 kDa) retain their IgE-binding capacity whereas smaller fragments (i.e., ≤ 12 kDa) do not
• Soluble CD23 may have immunoregulatory effects on the growth and differentiation of B cells and other cell types
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.