CD117 Mouse anti-Human, Brilliant Violet 650, Clone: YB5.B8, BD Optibuild™

Description

• The YB5.B8 monoclonal antibody specifically binds to CD117, which is also known as c-Kit, or Stem cell factor Receptor (SCFR)
• CD117 is a ∽145 kDa type I transmembrane glycoprotein with tyrosine kinase activity
• CD117 is present on hematopoietic progenitor cell subsets, thymocytes, mast cells, hepatocytes and histiocytes
• CD117 serves as a cytokine receptor for steel factor (SLF), also known as stem cell factor (SCF), or mast cell growth factor (MGF)
• The interaction of CD117 and SLF is crucial to hematopoiesis, mast cell differentiation, melanogenesis, and germ cell development
• The ability of the YB5.B8 antibody to block the binding of c-Kit ligand is still controversial
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.