CD7 Mouse anti-Human, Brilliant Violet 650, Clone: M-T701, BD Optibuild™

Description

• The M-T701 monoclonal antibody specifically binds to CD7
• CD7 is a 40 kDa type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily
• The CD7 antigen is also known as Leu-9, TP41, Tp40, GP40, and T-cell leukemia antigen
• It is expressed on thymocytes, T cells, pre-B cells, and NK cells
• CD7 is present in reduced density on monocytic cells and cell lines
• Functional studies demonstrate that crosslinking of the CD7 can induce transmembrane calcium flux in T cells
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.