CD23 Rat anti-Mouse, Brilliant Violet 650, Clone: B3B4, BD Optibuild™

Description

• The B3B4 monoclonal antibody specifically binds to CD23, the low affinity IgE Fc receptor (Fc ε RII) expressed on mature resting conventional B lymphocytes, but not on B-1 cells (CD5+ B cells) or T lymphocytes
• It does not react with high-affinity IgE receptors, as demonstrated on mouse mast cell lines
• The regulation of CD23 surface expression on activated B cells appears to be complex, depending upon the mode of activation and the presence of cytokines
• IgE synthesis is negatively regulated by CD23, and CD23 expression is upregulated on splenocytes in the presence of IgE
• CD23 is also upregulated on follicular dendritic cells in the lymph nodes of immunized mice, and a subset of splenic dendritic cells expresses CD23
• The B3B4 antibody abrogates antigen-specific IgE-dependent modulation of immune responses in normal mice
• This monoclonal antibody also blocks IgE binding and eosinophil infiltration in the lung of immunized mice
• Different in vivo results have been obtained when using the intact B3B4 antibody or the F(ab')2 fragments
• B3B4 mAb does not cross-react with rat or human IgE Fc Receptor
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.