CD197 (CCR7) Rat anti-Mouse, Brilliant Violet 605, Clone: 4B12, BD Optibuild™

Description

• The monoclonal antibody 4B12/CCR7 reacts with the mouse C-C chemokine receptor type 7 (CCR7)
• CCR7 is also known as CD197 (previously known as EBI1, Ebi1h and CMKBR7) and plays a central role in mediating homeostatic B and T lymphocyte trafficking to and within secondary lymphoid tissues
• CD197 is a seven-transmembrane, G-protein-coupled, 43 kDa glycoprotein receptor that is specific for the CC chemokines, MIP3ß/Exodus-3/ELC/CKb11/Scya19/CCL19 and 6Ckine/Exodus-2/SLC/TCA4/CKb9/Scya21/CCL21
• The mouse Ccr7 gene is located on chromosome 11
• CD197 (CCR7) is differentially expressed by subsets of thymocytes
• Positive CD197 expression appears to be involved in the cortex-to-medulla migration of positively-selected thymocytes wherein they complete functional maturation including the establishment of central tolerance
• It is most highly expressed by some mature medullary single-positive thymocytes
• CD197 is also expressed by subsets of mature peripheral CD4+ and CD8+ T lymphocytes including naοve and regulatory T cells and central memory T cells
• In addition, it is differentially expressed by subsets of B lymphocytes, dendritic cells, and Langerhans cells
• CD197 serves as a homing receptor that helps guide these various cell types to and within lymphoid tissues
• In this way, CCR7 supports protective immunity while safeguarding self tolerance
• Reportedly, the 4B12/CCR7 antibody is not agonistic, is not blocked by CCL21 nor by physiologic levels of CCL19, nor does the antibody block the binding of CCL21 to CCR7
• The immunogen used to generate the 4B12 hybridoma was a mouse CCR7-transfected rat cell line
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.