HLA-DR, DP, DQ Mouse anti-Human, Brilliant Violet 650, Clone: Tu39 (also known as Tü39), BD Optibuild™

Description

• The TU39 monoclonal antibody specifically recognizes human major histocompatibility (MHC) Class II HLA-DR, DP and most DQ antigens
• These antigens are encoded by genes within the Human Leukocyte Antigen (HLA) Complex located on chromosome 6
• MHC Class II antigens are transmembrane heterodimeric glycoproteins composed of α chain (36 kDa) and β chain (27 kDa) subunits
• They are expressed primarily on antigen presenting cells which include dendritic cells, monocytes, macrophages, thymic epitheli al cells, and B cells
• They are also expressed on activated T cells
• This molecule plays a major role in mediating cellular interactions during antigen presentation to CD4+ T lineage cells
• The TU39 antibody is reportedly useful for immunophenotyping as well as functional studies including the inhibition of mixed lymphocyte reactions and antibody-mediated complement fixation on target cells
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.