HLA-DR, DP, DQ Mouse anti-Human, Brilliant Violet 605, Clone: Tu39 (also known as Tü39), BD Optibuild™

Description

• The TU39 monoclonal antibody specifically recognizes human major histocompatibility (MHC) Class II HLA-DR, DP and most DQ antigens
• These antigens are encoded by genes within the Human Leukocyte Antigen (HLA) Complex located on chromosome 6
• MHC Class II antigens are transmembrane heterodimeric glycoproteins composed of α chain (36 kDa) and β chain (27 kDa) subunits
• They are expressed primarily on antigen presenting cells which include dendritic cells, monocytes, macrophages, thymic epitheli al cells, and B cells
• They are also expressed on activated T cells
• This molecule plays a major role in mediating cellular interactions during antigen presentation to CD4+ T lineage cells
• The TU39 antibody is reportedly useful for immunophenotyping as well as functional studies including the inhibition of mixed lymphocyte reactions and antibody-mediated complement fixation on target cells
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.