CD71 Rat anti-Mouse, BB700, Clone: C2 (also known as C2F2), BD Optibuild™

Description

• The C2 monoclonal antibody specifically binds to CD71, the transferrin receptor
• CD71 is a disulfide-linked homodimer of 95-kDa subunits
• CD71 mediates one of the cellular mechanisms for iron uptake, and its expression is regulated according to the cell's iron requirements
• It is expressed at high levels on developing erythroid cells, and it is upregulated after mitogenic activation of B or T lymphocytes
• The C2 monoclonal antibody selectivity inhibits some types of T- and B-cell activation by down-regulation of transferrin receptor expression, but it does not block binding of transferrin
• The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes
• It is a polymer-based tandem dye developed exclusively by BD Biosciences
• With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40nm filter)
• This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.BD Horizon Brilliant™ Blue 700 (BB700) is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5
• This dye also has less cross laser excitation off the 405 nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.  Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously.