CD9 Rat anti-Mouse, BB700, Clone: KMC8 (also known as KMC 8), BD Optibuild™

Description

• The KMC8 monoclonal antibody specifically binds to CD9 which is also known as Tetraspanin-29 (Tspan29)
• CD9 is a 24-kDa member of the transmembrane 4 superfamily, also called the tretraspanin family
• In the mouse, CD9 is present on bone marrow myeloid cells, stromal cells, and megakaryocyte-committed progenitors; subsets of peripheral T and B lymphocytes; and neutrophils, platelets, dendritic cells, and bone marrow-derived macrophages
• CD9 has been found to be associated with integrins and other cell-surface receptors
• It appears to play roles in signal transduction and in regulating cellular adhesive properties
• CD9 can also reportedly participate in T-cell costimulation and induction of apoptosis
• The KMC8 antibody reportedly blocks certain CD9 functions and activates macrophages
• The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes
• It is a polymer-based tandem dye developed exclusively by BD Biosciences
• With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40nm filter)
• This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.BD Horizon Brilliant™ Blue 700 (BB700) is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5
• This dye also has less cross laser excitation off the 405 nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.  Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously.