Siglec-1 (CD169) Mouse anti-Human, Brilliant Violet 421, Clone: 7-239, BD Optibuild™

Description

• The 7-239 monoclonal antibody specifically binds to Sialic acid-binding Ig-like lectin 1 (Siglec-1), which is also known as Sialoadhesin (SN), or CD169
• Siglec-1 is a type I transmembrane glycoprotein that belongs to the Siglec family within the Ig superfamily
• This adhesion molecule especiallybinds to glycolipids and glycoproteins with terminal α-2 sialyl residues.Siglec-1 is expressed by macrophages and dendritic cells and serves as a cellular interaction molecule
• Its expression can be upregulated by cells in response to type II collagen, or to cytokines including interferons, and tumor necrosis factor
• Siglec-1 plays roles in endocytosis, hematopoiesis, and leucocyte migration
• It mediates macrophage binding to various cell types including developing and mature leucocytes
• Siglec-1 that is expressed by dendritic cells can also bind HIV-1 and may mediate viral transfer to bystander CD4+ T cells
• Several Siglec-1 counter-receptors have been described including CD43, CD206, and CD227 which are expressed by T cells, macrophages, or breast cancer cells, respectively
• The 7-239 antibody reportedly blocks Siglec-1 functions in some cellular assay systems
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.