CD91 Mouse anti-Human, Brilliant Violet 510, Clone: A2MR-α2, BD Optibuild™

Description

• The A2MR-α2 monoclonal antibody specifically reacts with a 600 kDa, type I membrane single protein, also known as theα2 Macroglobulin (α2M) receptor/low density lipoprotein receptor-related protein 1 (LRP-1)
• Reported to be an endocytic receptor involved with intracellular signalling, lipid homeostasis, clearance of apoptotic cells, andα2 Macroglobulin mediated clearance of secreted amyloid precursor protein found in Alzheimer patients
• The single chain receptor undergoes cleavage, shortly after synthesis, into the 85 kDa transmembraneβchain that non-covalently binds to the extracellular 500-515 kDa a chain
• It has a broad cellular distribution, but in the hematopoietic system it is expressed on monocyte lineage cells.α2M/LRP-1 mediates endocytosis of a variety of ligands includingα2M-proteinase complexes, plasminogen activators in complex with plasminogen activator inhibitor, or Pseudomonas /Exotoxin A
• Ligand binding toα2M/LRP-1 is followed by rapid transport of the ligand to lysosomes for degradation
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set
• BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser
• BV510 is a polymer-based dye that is brighter than BD Horizon V500
• Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.