CD91 Mouse anti-Human, Brilliant Violet 711, Clone: A2MR-α2, BD Optibuild™

Description

• The A2MR-α2 monoclonal antibody specifically reacts with a 600 kDa, type I membrane single protein, also known as theα2 Macroglobulin (α2M) receptor/low density lipoprotein receptor-related protein 1 (LRP-1)
• Reported to be an endocytic receptor involved with intracellular signalling, lipid homeostasis, clearance of apoptotic cells, andα2 Macroglobulin mediated clearance of secreted amyloid precursor protein found in Alzheimer patients
• The single chain receptor undergoes cleavage, shortly after synthesis, into the 85 kDa transmembraneβchain that non-covalently binds to the extracellular 500-515 kDa a chain
• It has a broad cellular distribution, but in the hematopoietic system it is expressed on monocyte lineage cells.α2M/LRP-1 mediates endocytosis of a variety of ligands includingα2M-proteinase complexes, plasminogen activators in complex with plasminogen activator inhibitor, or Pseudomonas /Exotoxin A
• Ligand binding toα2M/LRP-1 is followed by rapid transport of the ligand to lysosomes for degradation
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.