CD91 Mouse anti-Human, Brilliant Violet 605, Clone: A2MR-α2, BD Optibuild™

Description

• The A2MR-α2 monoclonal antibody specifically reacts with a 600 kDa, type I membrane single protein, also known as theα2 Macroglobulin (α2M) receptor/low density lipoprotein receptor-related protein 1 (LRP-1)
• Reported to be an endocytic receptor involved with intracellular signalling, lipid homeostasis, clearance of apoptotic cells, andα2 Macroglobulin mediated clearance of secreted amyloid precursor protein found in Alzheimer patients
• The single chain receptor undergoes cleavage, shortly after synthesis, into the 85 kDa transmembraneβchain that non-covalently binds to the extracellular 500-515 kDa a chain
• It has a broad cellular distribution, but in the hematopoietic system it is expressed on monocyte lineage cells.α2M/LRP-1 mediates endocytosis of a variety of ligands includingα2M-proteinase complexes, plasminogen activators in complex with plasminogen activator inhibitor, or Pseudomonas /Exotoxin A
• Ligand binding toα2M/LRP-1 is followed by rapid transport of the ligand to lysosomes for degradation
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.