TSLP Receptor Mouse anti-Human, Brilliant Violet 650, Clone: 1F11/TSLPR (1F11 and AB81_85.1F11), BD Optibuild™

Description

• The 1F11/TSLPR mAb specifically binds to Thymic Stromal Lymphopoietin Receptor (TSLPR)
• TSLPR is a member of the hematopoietin receptor superfamily and is also known as Cytokine Receptor-like Factor 2
• The functional TSLPR complex consists of two subunits, TSLPR and the alpha subunit of the Interleukin-7 Receptor (IL-7Rα)
• Analysis of the TSLPR reveals sequence similarity with the common cytokine receptor gamma chain
• Functional TSLPRs are expressed by epithelial cells and a variety of hematopoietic cell types, including thymocytes, T cells, B cells, natural killer T cells, monocytes, macrophages, basophils, and dendritic cells (DC)
• Recent studies indicate that TSLP can activate multiple STAT signaling proteins
• TSLP enhances the maturation and viability of DC
• It strongly induces DC expression of the CD40 and CD80 costimulatory molecules and chemokines, e.g., TARC (Thymus and activation-regulated chemokine; CCL17) that can attract Th2 effector cells
• TSLP supports B cell development
• TSLP costimulates the proliferation of naοve T cells in the presence of mature DC
• TSLP is also able to increase the sensitivity of T cell receptor-activated CD4+ T cells to low doses of IL-2
• In the presence of TSLP, the acute myeloid leukemia-derived cell line, MUTZ-3, shows induced growth and reduced apoptosis
• CRLF2 deregulated gene expression is thought to be involved in lymphoid transformation in B-cell precursor acute lymphoblastic leukemia
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.