CD161a Mouse anti-Rat, BUV395, Clone: 10/78., BD Optibuild™

Description

• The 10/78 monoclonal antibody recognizes the rat CD161 proteins, CD161a (also known as, Klrb1a, or Nkrp1a/NKR-P1A), and CD161b (Klrb1b, Nkrp1b/NKR-P1B)
• These type II transmembrane glycoproteins have an extracellular C-type lectin domain and thus belong to the C-type lectin superfamily
• These CD161 proteins form ñ 60 kDa homodimers that are expressed on natural killer cells and subsets of T lymphocytes, activated monocytes, and dendritic cells
• The 10/78 antibody competes with the previously-described 3.2.3 antibody for binding to these CD161 proteins
• CD161 molecules are C-type lectin-like receptors that can either activate (CD161a) or inhibit (CD161b) effector leucocyte responses, eg, cytotoxicity or cytokine production, against target cells which express C-type lectin-like related (Clr) molecules
• Although several members of the Klrb1 /gene family have been identified in the mouse and rat, only a single human KLRB1 /homolog has been discovered The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.