CD11c Hamster anti-Mouse, BUV395, Clone: N418, BD Optibuild™

Description

• The N418 monoclonal antibody specifically binds to CD11c, the Integrin alpha X (Integrin X, Itgax) chain of the heterodimeric gp150, 95 (CD11c/CD18, ) integrin that forms the complement receptor 4 (CR4)
• CD11c is a150 kDa type I transmembrane glycoprotein that is expressed on dendritic cells, CD4-CD8+ intestinal intraepithelial lymphocytes (IEL), and some NK cells and T cells
• CD11c expression is upregulated on IEL and T cells following activation
• Cells of the monocyte/macrophage lineage have been reported to express low levels of CD11c
• The CD11c/CD18 integrin can bind to several ligands including iC3b, fibrinogen, and CD54
• This integrin reportedly plays important roles in phagocytosis and in mediating cellular interactions during inflammation
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.