EphB2 Mouse, anti-Human, R718, Clone: 2H9, BD OptiBuild™

Description

• The 2H9 monoclonal antibody specifically binds to the Ephrin Type-B Receptor 2 (EphB2)
• EphB2 is a type I transmembrane glycoprotein that belongs to the Eph receptor family of tyrosine kinase receptors
• EphB2 serves as a cell surface receptor tyrosine kinase for membrane-anchored ligands referred to as type B ephrins (ephrin-B)
• The EphB2 receptor can bind to ephrin-B1, ephrin-B2, and ephrin-B3
• Transmembrane ephrin-B family members are key regulators of embryogenesis including development of the nervous and vascular systems
• The EphB2 receptor functions as a chemodirectant in regulating cellular migration
• EphB2/ephrin-B interactions orchestrate cell positioning by regulating cellular adhesion and repulsion during development, thereby influencing cell fate, morphogenesis and organogenesis
• Signaling can occur in a forward pathway when the EphB2 receptor tyrosine kinase is activated by bound ligand and in a reverse pathway when transmembrane ephrin-B ligands are activated by EphB2 receptor-mediated crosslinking
• In the adult body, Eph receptor signaling plays major roles in regulating the architecture and physiology of different tissues under normal as well as disease conditions such as cancer
• Ephrin-B1 and ephrin-B2 levels are upregulated in the vasculature during inflammation
• Ephrin-B2 molecules that are localized to the luminal endothelial surface can signal through the EphB2 which is expressed by monocytes
• This interaction promotes monocyte differentiation into proinflammatory macrophages
• In the intestinal epithelium, EphB2/ephrin-B interactions regulate both cell positioning and tumor progression
• The differential expression patterns of EphB2 allows for the detection and isolation of various intestinal epithelial cell types
• These include intestinal stem cells (ISCs) which express high levels of EphB2
• The 2H9 antibody reportedly blocks the interaction of EphB2 with ephrin ligands and crossreacts with mouse EphB2.