Lgr5 Mouse, anti-Human, R718, Clone: 8F2, BD OptiBuild™

Description

• Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) is a seven transmembrane-domain receptor that is a target gene for Wnt and marks stem cells in the small intestine, colon, stomach, and hair follicle
• Lgr5 was initially identified as a potential stem cell marker due to restricted expression of Lgr5 in the intestinal crypt and labeling of rapidly cycling cells of the colon and intestine
• Using both lineage tracing and organoid culture experiments, Lgr5 positive cells are capable of generating all types of the small intestine epithelium hence indicating that Lgr5 marks stem cells of the small intestine and colon
• R-spondin growth factors, which are secreted agonists of the Wnt pathway, bind Lgr5
• The binding of R-spondins to Lgr5 leads to recruitment of the Frizzled/LRP Wnt receptor complex, which binds to Wnt ligands and leads to downstream Wnt signaling
• Lgr5 is up-regulated in colon and ovarian cancers and has been implicated in promotion of tumor growth and metastasis
• The 8F2 monoclonal antibody recognizes an epitope in the N-terminal region of Human Lgr5.