Phospho-ATM (Ser1981) Monoclonal Antibody (10H11), Invitrogen™

Description

• MA1-2020 detects the phospho-ATM kinase in human and mouse samples
• MA1-2020 has been successfully used in immunofluorescence, immunoprecipitation and Western blot procedures
• By Western blot this antibody detects a ∽370 kDa protein representing the phospho-ATM kinase in crude lysates from gamma irradiated HeLa cells
• In immunofluorescence procedures, MA1-2020 recognizes the phospho-ATM kinase in irradiated human and mouse fibroblasts
• The MA1-2020 immunogen is a phosphorylated synthetic peptide corresponding to the residues S(1974) L A F E E S(p) Q S T T I S S(1988) of human ATM Kinase protein
• Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family
• Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer
• The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily
• ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1
• The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens
• The ATM’s C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases)
• Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation
• AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction
• Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair
• Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.