Phospho-Histone H2A.X (Ser139) Monoclonal Antibody (3F2), Invitrogen™

Description

• MA1-2022 was used in IHC to successfully detect H2A.X pSer140 in postnatal mouse lung section
• MA1-2022 detects human and mouse phosphorylated H2AX
• MA1-2022 has been used successfully in Western blot, immunofluorescence, and ELISA procedures
• By Western blot this antibody detects ∽17 kDa protein representing phosphorylated H2AX in gamma irradiated HeLa cell lysate
• In immunofluorescence procedures, MA1-2022 recognizes phosphorylated H2AX in gamma irradiated HeLa cells
• ELISA of phosphorylated H2AX can also be performed using MA1-2022
• MA1-2022 was used in IHC to successfully detect H2A.X pSer140 in postnatal mouse lung section
• The MA1-2022 immunogen is a synthetic peptide sequence surrounding phosphorylated Ser140
• Some references call Ser140, by the name Ser139
• This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit
• The antibody sold separately may have slightly different performance and may need to be further optimized for the best results
• Histone H2A.X (H2AX) is a member of the histone H2A family which is one of the four core histones making up the nucleosome core particle
• In eukaryotes, DNA double strand breaks (DSBs) have been shown to trigger the phosphorylation of serine 139 at the carboxy terminus of histone H2AX resulting in gamma-H2AX
• The phosphorylation of H2AX can be detected by Western blotting or immunofluorescence, revealing the frequency of DSBs
• The phosphatidylinositol 3-kinases have been implicated in H2AX phosphorylation, but it is unclear if ATM is the primary H2AX kinase or if other members of the family such as DNA-PK and ATR contribute in a similar manner
• Structurally, H2A.x contains 143 amino acid residues
• Histone H2A.X is considered a basal histone, being synthesized in G1 as well as in S-phase, and its mRNA contains polyA addition motifs and a polyA tail along with the conserved stem-loop and U7 binding sequences involved in the processing and stability of replication type histone mRNAs
• There are two forms of Histone H2A.X mRNA, one about 1600 bases long and contains polyA; the other about 575 bases long, lacking polyA
• The short form behaves as a replication type histone mRNA, while the longer behaves as a basal type histone mRNA
• Histone H2A.X maps to the 11q23.2-q23.3 region of the human chromosome
• Histone H2A.x contributes to histone-formation and therefore the structure of DNA
• Histone H2A variant H2A.x specifically regulates the interaction of MDC1 (mediator of DNA damage checkpoint protein 1), a DNA repair protein to the sites of DNA damage.