alpha Adaptin Monoclonal Antibody (AC1-M11), Invitrogen™

Description

• MA3-061 detects alpha-adaptin from bovine, chicken, frog, human, monkey, mouse, pig and rat
• This antibody detects the products of both alpha-adaptin genes, alphaA and alphaC, as well as an alternatively spliced isoform of alphaA found in neurons
• MA3-061 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation procedures
• By Western blot, this antibody recognizes an ∽100 kDa doublet representing the two isoforms of alpha-adaptin from pig brain extract
• Immunofluorescence staining of alpha-adaptin in transfected COS cells with MA3-061A yields distinct punctate dots on the plasma membrane
• The MA3-061 antigen is purified bovine brain adaptor complexes
• Component of the adaptor protein complex 2 (AP-2)
• Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways
• Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation
• AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome
• The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components
• Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation
• AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis
• AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface
• AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules
• AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway
• The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane
• The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins
• The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.