MAB19562AF6
MilliporeSigma™ anti-Laminin-5 Alexa Fluor 647, Clone: D4B5,
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MAB19562AF6-Each-of-1 | In Stock | ₹ 48,852.19 |
MAB19562AF6 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 48,852.19
GST (18%): ₹ 8,793.394
Total Price: ₹ 57,645.584
Antigen
Laminin-5
Classification
Monoclonal
Conjugate
Alexa Fluor 647
Gene Accession No.
Q13753-1
Host Species
Mouse
Purification Method
Protein A purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This antibody recognizes the 150 (precursor form) and 105kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5.
Content And Storage
2°C to 8°C for one year from date of shipment
Isotype
IgG1
Applications
Immunocytochemistry
Clone
D4B5
Formulation
Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.
Gene Symbols
LAMC2, LAMB2T, LAMNB2
Immunogen
Amino acid residues 382-608 of human laminin-5 gamma2 chain (III domain) expressed as a GST fusion protein.
Quantity
100 μL
Research Discipline
Cell Structure
Gene ID (Entrez)
NP_005553
Target Species
Human, Mouse
Form
Purified
Related Products
Description
- Specifically detects Laminin-5 Clone: D4B5 in Human, Mouse samples, and it is validated for Immunocytochemistry Laminin subunit gamma-2 (UniProt Q13753; also known as CSF 140kDa subunit, Cell-scattering factor 140kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Ladsin 140kDa subunit, Laminin B2t chain, Lamin-5 subunit gamma, Large adhesive scatter factor 140kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as B2T, LAMB2T, LAMNB2) gene (Gene ID 3918) in human
- Laminins are basement membrane, extracellular matrix glycoproteins composed of alpha, beta and gamma heterotrimeric chains
- Different combinations of five alpha chains, three beta chains, and two gamma chains form at least 12 laminin isoforms
- Laminin-5 (LN5), which consists of laminin alpha3, beta3 and gamma2 chains, is a highly conserved component of anchoring filaments underlying the hemidesmosome structure of epidermal keratinocytes
- Mutations or deletion in the LN5 genes (LAMA3, LAMB3, and LAMC2) is associated with epidermolysis bullosa, a lethal skin blistering disease
- LN5 may stabilize the epidermal/dermal junction of skin through interaction with integrins alpha3/beta1, alpha6/beta4 and type VII collagen and has a critical role in the anchorage and locomotion of a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes
- Comparing to fibronectin, collagen, and vitronectin, cells of epithelial origin will adhere to Laminin-5 faster and will spread to a larger extent
- Laminin-5 attracted attention when it was detected at the leading edge of invasive cancer tissue in several types of human carcinomas (Niki, T., et al
- (2002)
- Am
- J
- Pathol
- 160(3):112911-41; Lohi, J
- (2001)
- Int
- J
- Cancer
- 94(6):763-767).
Compare Similar Items
Show Difference
Antigen: Laminin-5
Classification: Monoclonal
Conjugate: Alexa Fluor 647
Gene Accession No.: Q13753-1
Host Species: Mouse
Purification Method: Protein A purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This antibody recognizes the 150 (precursor form) and 105kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5.
Content And Storage: 2°C to 8°C for one year from date of shipment
Isotype: IgG1
Applications: Immunocytochemistry
Clone: D4B5
Formulation: Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.
Gene Symbols: LAMC2, LAMB2T, LAMNB2
Immunogen: Amino acid residues 382-608 of human laminin-5 gamma2 chain (III domain) expressed as a GST fusion protein.
Quantity: 100 μL
Research Discipline: Cell Structure
Gene ID (Entrez): NP_005553
Target Species: Human, Mouse
Form: Purified
Antigen:
Laminin-5
Classification:
Monoclonal
Conjugate:
Alexa Fluor 647
Gene Accession No.:
Q13753-1
Host Species:
Mouse
Purification Method:
Protein A purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
This antibody recognizes the 150 (precursor form) and 105kDa (mature form) chain proteins of Laminin-5. No reactivity is expected with other laminin types, as the gamma2 chain of laminin is unique to laminin-5.
Content And Storage:
2°C to 8°C for one year from date of shipment
Isotype:
IgG1
Applications:
Immunocytochemistry
Clone:
D4B5
Formulation:
Purified mouse antibody conjugate in PBS with 15mg/mL BSA and 0.1 % sodium azide.
Gene Symbols:
LAMC2, LAMB2T, LAMNB2
Immunogen:
Amino acid residues 382-608 of human laminin-5 gamma2 chain (III domain) expressed as a GST fusion protein.
Quantity:
100 μL
Research Discipline:
Cell Structure
Gene ID (Entrez):
NP_005553
Target Species:
Human, Mouse
Form:
Purified
Antigen: Laminin-5 (γ2 chain)
Classification: Monoclonal
Conjugate: Unconjugated
Gene Accession No.: NM_005562.2; NM_018891.2
Host Species: Mouse
Purification Method: Protein A purfied
Regulatory Status: __
Primary or Secondary: Primary
Test Specificity: __
Content And Storage: 2-8°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Isotype: IgG1
Applications: ELISA, Immunohistochemistry, Western Blot
Clone: D4B5
Formulation: Protein A Purified mouse immunoglobulin in 20mM sodium phosphate, 250mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Gene Symbols: LAMC2, EBR2, LAMB2T, MGC141938, MGC138491, LAMNB2, epiligrin, BM600-100kDa, BM600, kalinin-105kDa, nicein-100kDa, EBR2A, B2T, CSF
Immunogen: Amino acid residues 382-608 of human laminin-5 gamma2 chain (III domain) expressed as a GST fusion protein
Quantity: 100 μg
Research Discipline: __
Gene ID (Entrez): __
Target Species: Human
Form: Purified
Antigen:
Laminin-5 (γ2 chain)
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Accession No.:
NM_005562.2; NM_018891.2
Host Species:
Mouse
Purification Method:
Protein A purfied
Regulatory Status:
__
Primary or Secondary:
Primary
Test Specificity:
__
Content And Storage:
2-8°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Isotype:
IgG1
Applications:
ELISA, Immunohistochemistry, Western Blot
Clone:
D4B5
Formulation:
Protein A Purified mouse immunoglobulin in 20mM sodium phosphate, 250mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Gene Symbols:
LAMC2, EBR2, LAMB2T, MGC141938, MGC138491, LAMNB2, epiligrin, BM600-100kDa, BM600, kalinin-105kDa, nicein-100kDa, EBR2A, B2T, CSF
Immunogen:
Amino acid residues 382-608 of human laminin-5 gamma2 chain (III domain) expressed as a GST fusion protein
Quantity:
100 μg
Research Discipline:
__
Gene ID (Entrez):
__
Target Species:
Human
Form:
Purified
Antigen: __
Classification: __
Conjugate: __
Gene Accession No.: __
Host Species: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Test Specificity: __
Content And Storage: __
Isotype: __
Applications: __
Clone: __
Formulation: __
Gene Symbols: __
Immunogen: __
Quantity: __
Research Discipline: __
Gene ID (Entrez): __
Target Species: __
Form: __
Antigen:
__
Classification:
__
Conjugate:
__
Gene Accession No.:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Test Specificity:
__
Content And Storage:
__
Isotype:
__
Applications:
__
Clone:
__
Formulation:
__
Gene Symbols:
__
Immunogen:
__
Quantity:
__
Research Discipline:
__
Gene ID (Entrez):
__
Target Species:
__
Form:
__
Antigen: Crossveinless-2/CV-2/BMPER
Classification: Monoclonal
Conjugate: Unconjugated
Gene Accession No.: Q8N8U9
Host Species: Rat
Purification Method: Protein A or G purified from hybridoma culture supernatant
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Detects human Crossveinless-2/CV-2 in direct ELISAs and Western blots. In Western blots, 25% cross-reactivity with recombinant mouse CV-2 is observed.
Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Isotype: IgG2a
Applications: Western Blot
Clone: 355304
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Symbols: BMPER
Immunogen: Mouse myeloma cell line NS0-derived recombinant human Crossveinless-2/CV-2 Val34-Arg685 Accession # Q8N8U9
Quantity: 25 μg
Research Discipline: __
Gene ID (Entrez): 168667
Target Species: Human
Form: Purified
Antigen:
Crossveinless-2/CV-2/BMPER
Classification:
Monoclonal
Conjugate:
Unconjugated
Gene Accession No.:
Q8N8U9
Host Species:
Rat
Purification Method:
Protein A or G purified from hybridoma culture supernatant
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Detects human Crossveinless-2/CV-2 in direct ELISAs and Western blots. In Western blots, 25% cross-reactivity with recombinant mouse CV-2 is observed.
Content And Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Isotype:
IgG2a
Applications:
Western Blot
Clone:
355304
Formulation:
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Symbols:
BMPER
Immunogen:
Mouse myeloma cell line NS0-derived recombinant human Crossveinless-2/CV-2 Val34-Arg685 Accession # Q8N8U9
Quantity:
25 μg
Research Discipline:
__
Gene ID (Entrez):
168667
Target Species:
Human
Form:
Purified