MABE1081
MilliporeSigma™ 5-Methylcytosine (5mC), Mouse, Unlabeled, Clone: EDL MC-4,
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE1081-Each-of-1 | In Stock | ₹ 37,453.03 |
MABE1081 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 37,453.03
GST (18%): ₹ 6,741.545
Total Price: ₹ 44,194.575
Antigen
5-Methylcytosine (5mC)
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse IgG2a in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen
BSA-conjugated 5-methylcytosine (5mC).
Quantity
100 μg
Research Discipline
Epigenetics & Nuclear Function
Test Specificity
Clone EDL MC-4 targets 5-methylcytosine (5mC), but not 5-hydroxymethylcytosine (5hmC), 5-carboxylcytosine (5caC), or unmodified cytosine (C). A less than 25% cross-reactivity toward 5-formylcytosine (5fC) was observed, which has no impact on 5mC detection by clone EDL MC-4 in cell samples, where 5mC modification level is typically more than 100- to 1,000-fold of 5fC modification level.
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG2a λ
Applications
Immunoprecipitation
Clone
EDL MC-4
Conjugate
Unconjugated
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
All species
Form
Purified
Related Products
Description
- Four types of cytosine modifications have been identified in mammalian DNA, namely 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC)
- While 5mC, 5hmC, and 5fC occur in embryonic stem (ES) cells and many cell types in all the major organs (e.g
- brain, heart, lung and kidney), 5caC has only been detected in a few cell types at a 10 times lower level than 5fC
- The sequential oxidation of 5mC to 5hmC, 5fC, and 5caC catalyzed by the ten-eleven translocation (TET) enzymes has been proposed as a potential route for the demethylation of 5mC back to cytosine (C)
- Thymine DNA glycosylase (TDG), for example, can excise 5fC and 5caC from DNA as part of the base excision repair (BER) pathway to restore the C-nucleotide
- Emerging evidences suggest that the different modifications may be selectively recognized by various proteins, and methyl-CpG-binding protein 2 (MeCP2) binding to 5hmC has been proposed as a means of epigenetic regulation in neurons
- The conversion of 5hmC to 5fC, in turn, induces an enhanced binding of DNA-repair-associated proteins to 5fC, which has been proposed as a signal for DNA demethylation.
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Antigen: 5-Methylcytosine (5mC)
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgG2a in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen: BSA-conjugated 5-methylcytosine (5mC).
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: Clone EDL MC-4 targets 5-methylcytosine (5mC), but not 5-hydroxymethylcytosine (5hmC), 5-carboxylcytosine (5caC), or unmodified cytosine (C). A less than 25% cross-reactivity toward 5-formylcytosine (5fC) was observed, which has no impact on 5mC detection by clone EDL MC-4 in cell samples, where 5mC modification level is typically more than 100- to 1,000-fold of 5fC modification level.
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG2a λ
Applications: Immunoprecipitation
Clone: EDL MC-4
Conjugate: Unconjugated
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: All species
Form: Purified
Antigen:
5-Methylcytosine (5mC)
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse IgG2a in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen:
BSA-conjugated 5-methylcytosine (5mC).
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
Clone EDL MC-4 targets 5-methylcytosine (5mC), but not 5-hydroxymethylcytosine (5hmC), 5-carboxylcytosine (5caC), or unmodified cytosine (C). A less than 25% cross-reactivity toward 5-formylcytosine (5fC) was observed, which has no impact on 5mC detection by clone EDL MC-4 in cell samples, where 5mC modification level is typically more than 100- to 1,000-fold of 5fC modification level.
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG2a λ
Applications:
Immunoprecipitation
Clone:
EDL MC-4
Conjugate:
Unconjugated
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
All species
Form:
Purified
Antigen: __
Classification: __
Concentration: __
Formulation: __
Immunogen: __
Quantity: __
Research Discipline: __
Test Specificity: __
Content And Storage: __
Isotype: __
Applications: __
Clone: __
Conjugate: __
Host Species: __
Purification Method: __
Regulatory Status: __
Primary or Secondary: __
Target Species: __
Form: __
Antigen:
__
Classification:
__
Concentration:
__
Formulation:
__
Immunogen:
__
Quantity:
__
Research Discipline:
__
Test Specificity:
__
Content And Storage:
__
Isotype:
__
Applications:
__
Clone:
__
Conjugate:
__
Host Species:
__
Purification Method:
__
Regulatory Status:
__
Primary or Secondary:
__
Target Species:
__
Form:
__
Antigen: 5-Hydroxymethylcytosine (5hmC)
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgG2b in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen: BSA-conjugated 5-hydroxymethylcytidine.
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: Clone EDL HMC 1A targets 5-hydroxymethylcytosine (5hmC), but not 5-methylcytosine (5mC), 5-carboxylcytosine (5caC), 5-formylcytosine (5fC), or unmodified cytosine (C).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG2b κ
Applications: Immunoprecipitation
Clone: EDL HMC 1A
Conjugate: Unconjugated
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: All species
Form: Purified
Antigen:
5-Hydroxymethylcytosine (5hmC)
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse IgG2b in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen:
BSA-conjugated 5-hydroxymethylcytidine.
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
Clone EDL HMC 1A targets 5-hydroxymethylcytosine (5hmC), but not 5-methylcytosine (5mC), 5-carboxylcytosine (5caC), 5-formylcytosine (5fC), or unmodified cytosine (C).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG2b κ
Applications:
Immunoprecipitation
Clone:
EDL HMC 1A
Conjugate:
Unconjugated
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
All species
Form:
Purified
Antigen: Cdx1
Classification: Monoclonal
Concentration: __
Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Immunogen: Linear peptide corresponding to a sequence from the N-terminal region of human Cdx1.
Quantity: 100 μg
Research Discipline: Epigenetics and Nuclear Function
Test Specificity: __
Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
Isotype: IgG1 κ
Applications: ChIP Assay, Immunocytochemistry, Western Blot
Clone: C
Conjugate: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
Cdx1
Classification:
Monoclonal
Concentration:
__
Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Immunogen:
Linear peptide corresponding to a sequence from the N-terminal region of human Cdx1.
Quantity:
100 μg
Research Discipline:
Epigenetics and Nuclear Function
Test Specificity:
__
Content And Storage:
Stable for one year at 2°C to 8°C from date of receipt.
Isotype:
IgG1 κ
Applications:
ChIP Assay, Immunocytochemistry, Western Blot
Clone:
C
Conjugate:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified