MABE1093MI
MilliporeSigma™ 5-Hydroxymethylcytosine (5hmC), Mouse, Unlabeled, Clone: EDL HMC 1A,
Manufacturer: MilliporeSigma™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABE1093MI-Each-of-1 | In Stock | ₹ 36,363.00 |
MABE1093MI - Each of 1
In Stock
Quantity
1
Base Price: ₹ 36,363.00
GST (18%): ₹ 6,545.34
Total Price: ₹ 42,908.34
Antigen
5-Hydroxymethylcytosine (5hmC)
Classification
Monoclonal
Concentration
Please refer to lot specific datasheet.
Formulation
Purified mouse IgG2b in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen
BSA-conjugated 5-hydroxymethylcytidine.
Quantity
100 μg
Research Discipline
Epigenetics & Nuclear Function
Test Specificity
Clone EDL HMC 1A targets 5-hydroxymethylcytosine (5hmC), but not 5-methylcytosine (5mC), 5-carboxylcytosine (5caC), 5-formylcytosine (5fC), or unmodified cytosine (C).
Content And Storage
Stable for 1 year at 2°-8°C from date of receipt.
Isotype
IgG2b κ
Applications
Immunoprecipitation
Clone
EDL HMC 1A
Conjugate
Unconjugated
Host Species
Mouse
Purification Method
Protein G Purified
Regulatory Status
RUO
Primary or Secondary
Primary
Target Species
All species
Form
Purified
Related Products
Description
- 5-Hydroxymethylcytosine (also known as 5-hmC) is the first oxidative product in the active demethylation of 5-methylcytosine (5mC)
- 5hmC is a DNA pyrimidine nitrogen base and is a predominantly stable DNA modification
- It is produced by the action of three Ten-eleven translocation 1 (TET1) enzyme on 5mC
- Each mammalian cell contains 5hmC, but its levels vary significantly depending on the cell type
- Higher levels of 5hmC are found in neuronal cells and its level is shown to increase with age in hippocampus and cerebellum
- It is also prevalent in embryonic stems cells and its reduced levels are shown to result in impaired self-renewal of stem cells
- In the brain and in embryonic stem cells, 5hmC in enriched in promoters, gene bodies, and intergenic areas near genes and positively correlates with gene expression at these loci
- 5hmC appears to play a role to promote gene expression during active demethylation
- Significantly lower of 5hmC have been reported in cancer cell lines, melanoma, adenomas, and carcinomas compared to the normal surrounding tissue.
Compare Similar Items
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Antigen: 5-Hydroxymethylcytosine (5hmC)
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgG2b in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen: BSA-conjugated 5-hydroxymethylcytidine.
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: Clone EDL HMC 1A targets 5-hydroxymethylcytosine (5hmC), but not 5-methylcytosine (5mC), 5-carboxylcytosine (5caC), 5-formylcytosine (5fC), or unmodified cytosine (C).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG2b κ
Applications: Immunoprecipitation
Clone: EDL HMC 1A
Conjugate: Unconjugated
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: All species
Form: Purified
Antigen:
5-Hydroxymethylcytosine (5hmC)
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse IgG2b in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen:
BSA-conjugated 5-hydroxymethylcytidine.
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
Clone EDL HMC 1A targets 5-hydroxymethylcytosine (5hmC), but not 5-methylcytosine (5mC), 5-carboxylcytosine (5caC), 5-formylcytosine (5fC), or unmodified cytosine (C).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG2b κ
Applications:
Immunoprecipitation
Clone:
EDL HMC 1A
Conjugate:
Unconjugated
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
All species
Form:
Purified
Antigen: Cdx1
Classification: Monoclonal
Concentration: __
Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Immunogen: Linear peptide corresponding to a sequence from the N-terminal region of human Cdx1.
Quantity: 100 μg
Research Discipline: Epigenetics and Nuclear Function
Test Specificity: __
Content And Storage: Stable for one year at 2°C to 8°C from date of receipt.
Isotype: IgG1 κ
Applications: ChIP Assay, Immunocytochemistry, Western Blot
Clone: C
Conjugate: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
Cdx1
Classification:
Monoclonal
Concentration:
__
Formulation:
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% sodium azide.
Immunogen:
Linear peptide corresponding to a sequence from the N-terminal region of human Cdx1.
Quantity:
100 μg
Research Discipline:
Epigenetics and Nuclear Function
Test Specificity:
__
Content And Storage:
Stable for one year at 2°C to 8°C from date of receipt.
Isotype:
IgG1 κ
Applications:
ChIP Assay, Immunocytochemistry, Western Blot
Clone:
C
Conjugate:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified
Antigen: DNA-RNA Hybrid
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgG2aκ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen: DNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG2a κ
Applications: ChIP Assay, Dot Blot, Immunocytochemistry, Immunoprecipitation
Clone: S9.6
Conjugate: Unconjugated
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: All species
Form: Purified
Antigen:
DNA-RNA Hybrid
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse IgG2aκ in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen:
DNA-RNA heteropolymer duplex prepared by transcription of phi X174 single-stranded DNA with DNA-dependent RNA polymerase (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
Clone S9.6 bound the DNA-RNA heteropolymer and poly(I)-poly(dC) equally, but 100-fold higher levels of poly(A)-poly(dT) were required to achieve a similar degree of binding. Single-stranded DNA, double-stranded DNA and RNA, and ribosomal RNA were not bound by clone S9.6 (Boguslawski, S.J., et al. (1986). J. Immunol Methods. 89(1):123-130).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG2a κ
Applications:
ChIP Assay, Dot Blot, Immunocytochemistry, Immunoprecipitation
Clone:
S9.6
Conjugate:
Unconjugated
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
All species
Form:
Purified
Antigen: SMARCA3 (HLTF)
Classification: Monoclonal
Concentration: Please refer to lot specific datasheet.
Formulation: Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen: GST-tagged recombinant human HLTF (SMARCA3) N-terminal fragment.
Quantity: 100 μg
Research Discipline: Epigenetics & Nuclear Function
Test Specificity: Clone 5-1 detected predominantly nuclear HLTF (SMARCA3) immunoreactivity by immunohistochemistry staining. Clone 5-1 epitope lies within an N-terminal region present in both spliced isoforms of human HLTF reported by UniProt (Q14527).
Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
Isotype: IgG1 κ
Applications: Immunohistochemistry (Paraffin)
Clone: 5-1
Conjugate: Unconjugated
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Target Species: Human
Form: Purified
Antigen:
SMARCA3 (HLTF)
Classification:
Monoclonal
Concentration:
Please refer to lot specific datasheet.
Formulation:
Purified mouse IgG1 in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
Immunogen:
GST-tagged recombinant human HLTF (SMARCA3) N-terminal fragment.
Quantity:
100 μg
Research Discipline:
Epigenetics & Nuclear Function
Test Specificity:
Clone 5-1 detected predominantly nuclear HLTF (SMARCA3) immunoreactivity by immunohistochemistry staining. Clone 5-1 epitope lies within an N-terminal region present in both spliced isoforms of human HLTF reported by UniProt (Q14527).
Content And Storage:
Stable for 1 year at 2°-8°C from date of receipt.
Isotype:
IgG1 κ
Applications:
Immunohistochemistry (Paraffin)
Clone:
5-1
Conjugate:
Unconjugated
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Target Species:
Human
Form:
Purified