MABN227525
phospho-Tau (Ser235), Mouse anti-Human, Mouse, Clone: RN235, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABN227525-Each-of-1 | In Stock | ₹ 16,456.61 |
MABN227525 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
phospho-Tau (Ser235)
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
MAPT;MAPTL;MTBT1;TAU
Immunogen
KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phospho serine 235 of human tau-C.
Quantity
25 μg
Research Discipline
Neuroscience
Gene ID (Entrez)
NP_001116538
Target Species
Human, Mouse
Form
Purified
Applications
Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Clone
RN235
Dilution
Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected phospho-tau (Ser235) in human Alzheimer's diseas (AD) brain tissue sections.Dot Blot analysis: A representative lot detected phosphorylated recombinant human tau (pSer235), but not the non-phosphorylated recombinant human tau (Tau). (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunofluorescence Analysis: A representative lot detected phospho-tau (Ser235) in K3 and rTg4510 tau transgenic mouse brains (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunohistochemistry (Paraffin) Analysis: A representative lot detected phospho-Tau (Ser235) in human Alzheimer s disease (AD) brain tissue sections. (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).ELISA Analysis: A representative lot full-length human recombinant phospho-tau (Ser235) or GSK-3 beta phosphorylated tau (pTau), but not the non-ph
Gene Alias
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone RN235 is a mouse monoclonal antibody that detects microtubule-associated protein tau (MAPT) phosphorylated on serine 235 of isoform Tau-C. It targets an epitope with in 12 amino acids surround pSer235. This sequence is also conserved in isoform Tau-F.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-phospho-Tau (Ser235), clone RN235, Cat
- No
- MABN2275, is a highly specific mouse monoclonal antibody that targets Microtubule-associated protein tau and has been tested for use in Dot Blot, ELISA, Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting
- Microtubule-associated protein tau (UniProt: P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID: 4137) in human
- Tau, a highly asymmetric and heat-stable protein, is expressed mainly in the brain, where it regulates the stability and orientation of microtubules in neurons, astrocytes, and oligodendrocytes
- Tau protein is reported to be the predominant component of the paired helical filaments (PHFs) and neurofibrillary tangles (NFTs) that are characteristic of pathological brain lesions in Alzheimer s disease
- The dephosphorylation of Tau protein promotes rapid and extensive microtubule polymerization, while the phosphorylation of Tau decreases its ability to promote microtubule assembly
- Tau is a substrate for a multitude of protein kinases, including CaM kinase II, casein kinase II, protein kinase A (PKA), MAP kinase (ERK2), Cdk5/ p35, and glycogen synthase kinase-3 (GSK-3) and is phosphorylated at many different sites in Alzheimer s disease brain
- It is believed that the phosphorylation of specific sites, rather than the overall extent of phosphorylation, is important in modulating the ability of Tau to bind microtubules and promote microtubule assembly
- This antibody (clone RN235) specifically reacts with tau phosphorylated at serine 235, and detects intraneuronal NFTs (iNFTs), and extraneuronal NFTs (eNFTs) in the brain tissue
- Although theoretically it can also react with phospho Thr231, but EC50 is very high compared to reactivity with phospho Ser235 (407 nM vs 0.61 nM)
- (Ref.: Brici, D., et al
- (2018)
- J
- Alzheimer s Dis
- 61(3); 899-905).
Compare Similar Items
Show Difference
Antigen: phospho-Tau (Ser235)
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: MAPT;MAPTL;MTBT1;TAU
Immunogen: KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phospho serine 235 of human tau-C.
Quantity: 25 μg
Research Discipline: Neuroscience
Gene ID (Entrez): NP_001116538
Target Species: Human, Mouse
Form: Purified
Applications: Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Clone: RN235
Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected phospho-tau (Ser235) in human Alzheimer's diseas (AD) brain tissue sections.Dot Blot analysis: A representative lot detected phosphorylated recombinant human tau (pSer235), but not the non-phosphorylated recombinant human tau (Tau). (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunofluorescence Analysis: A representative lot detected phospho-tau (Ser235) in K3 and rTg4510 tau transgenic mouse brains (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunohistochemistry (Paraffin) Analysis: A representative lot detected phospho-Tau (Ser235) in human Alzheimer s disease (AD) brain tissue sections. (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).ELISA Analysis: A representative lot full-length human recombinant phospho-tau (Ser235) or GSK-3 beta phosphorylated tau (pTau), but not the non-ph
Gene Alias: Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone RN235 is a mouse monoclonal antibody that detects microtubule-associated protein tau (MAPT) phosphorylated on serine 235 of isoform Tau-C. It targets an epitope with in 12 amino acids surround pSer235. This sequence is also conserved in isoform Tau-F.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
phospho-Tau (Ser235)
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
MAPT;MAPTL;MTBT1;TAU
Immunogen:
KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phospho serine 235 of human tau-C.
Quantity:
25 μg
Research Discipline:
Neuroscience
Gene ID (Entrez):
NP_001116538
Target Species:
Human, Mouse
Form:
Purified
Applications:
Dot Blot, ELISA, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Clone:
RN235
Dilution:
Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected phospho-tau (Ser235) in human Alzheimer's diseas (AD) brain tissue sections.Dot Blot analysis: A representative lot detected phosphorylated recombinant human tau (pSer235), but not the non-phosphorylated recombinant human tau (Tau). (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunofluorescence Analysis: A representative lot detected phospho-tau (Ser235) in K3 and rTg4510 tau transgenic mouse brains (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).Immunohistochemistry (Paraffin) Analysis: A representative lot detected phospho-Tau (Ser235) in human Alzheimer s disease (AD) brain tissue sections. (Courtesy of Rebecca Nisbet, Ph.D., University of Queensland, Brisbane, Austraila).ELISA Analysis: A representative lot full-length human recombinant phospho-tau (Ser235) or GSK-3 beta phosphorylated tau (pTau), but not the non-ph
Gene Alias:
Microtubule-associated protein tau;Neurofibrillary tangle protein;Paired helical filament-tau;PHF-tau
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone RN235 is a mouse monoclonal antibody that detects microtubule-associated protein tau (MAPT) phosphorylated on serine 235 of isoform Tau-C. It targets an epitope with in 12 amino acids surround pSer235. This sequence is also conserved in isoform Tau-F.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: Dab1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: Dab1
Immunogen: GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.
Quantity: 100 μL
Research Discipline: Neuroscience
Gene ID (Entrez): NP_034144.1
Target Species: Mouse
Form: Purified
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: 4E12
Dilution: Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).
Gene Alias: Disabled homolog 1
Host Species: Rat
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2a κ
Antigen:
Dab1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
Dab1
Immunogen:
GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.
Quantity:
100 μL
Research Discipline:
Neuroscience
Gene ID (Entrez):
NP_034144.1
Target Species:
Mouse
Form:
Purified
Applications:
Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
4E12
Dilution:
Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).
Gene Alias:
Disabled homolog 1
Host Species:
Rat
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2a κ
Antigen: Dab1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: Dab1
Immunogen: GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.
Quantity: 25 μL
Research Discipline: Neuroscience
Gene ID (Entrez): NP_034144.1
Target Species: Mouse
Form: Purified
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Clone: 4E12
Dilution: Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).
Gene Alias: Disabled homolog 1
Host Species: Rat
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG2a κ
Antigen:
Dab1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
Dab1
Immunogen:
GST-tagged full-length recombinant murine Disabled homolog 1 expressed in E. coli.
Quantity:
25 μL
Research Discipline:
Neuroscience
Gene ID (Entrez):
NP_034144.1
Target Species:
Mouse
Form:
Purified
Applications:
Immunohistochemistry, Immunoprecipitation, Western Blot
Clone:
4E12
Dilution:
Western Blotting Analysis: A representative lot detected Dab1 in Western Blotting applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27; Nakamura, K., et. al. (2016). Neuroscience. 336:20-29).Immunoprecipitation Analysis: A 1:500 dilution from a representative lot immunoprecipitated Dab1 in cerebral cortex tissue lysate from P20 mouse (Courtesy of Prof. Mitsuharu Hattori, Nagoya City University).Immunohistochemistry Analysis: A representative lot detected Dab1 in Immunohistochemistry applications (Onoune, A., et. al. (2014). Neurosci Res. 88:23-27).
Gene Alias:
Disabled homolog 1
Host Species:
Rat
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 4E12 is a rat monoclonal antibody that detects murine Disabled homolog 1 (Dab1).
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG2a κ
Antigen: FMR1polyG
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: __
Gene Symbols: FMR1
Immunogen: Ovalbumin-conjugated linear peptide with EAPLPGGVRQRGG sequence. Note: This protein is translated within the 5'UTR of the FMR1 gene and in a different frame resulting in a protein with different sequence and containing polyGlycine.
Quantity: 100 μL
Research Discipline: Neuroscience
Gene ID (Entrez): NP_002015
Target Species: Human
Form: Ascites
Applications: Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Clone: 8FM-2F7
Dilution: __
Gene Alias: __
Host Species: Mouse
Purification Method: Unpurified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 8FM-2F7 specifically detects FMR1polyG in human cells.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG1 κ
Antigen:
FMR1polyG
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
__
Gene Symbols:
FMR1
Immunogen:
Ovalbumin-conjugated linear peptide with EAPLPGGVRQRGG sequence. Note: This protein is translated within the 5'UTR of the FMR1 gene and in a different frame resulting in a protein with different sequence and containing polyGlycine.
Quantity:
100 μL
Research Discipline:
Neuroscience
Gene ID (Entrez):
NP_002015
Target Species:
Human
Form:
Ascites
Applications:
Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
Clone:
8FM-2F7
Dilution:
__
Gene Alias:
__
Host Species:
Mouse
Purification Method:
Unpurified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 8FM-2F7 specifically detects FMR1polyG in human cells.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype:
IgG1 κ