MABS202625
EphA2, Mouse anti-Human, Clone: F2-27, Millipore Sigma™
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | MABS202625-Each-of-1 | In Stock | ₹ 16,456.61 |
MABS202625 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 16,456.61
GST (18%): ₹ 2,962.19
Total Price: ₹ 19,418.80
Antigen
EphA2
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols
EPHA2;ECK
Immunogen
KP-2, KP-3, SUIT-2, and MIAPaCa-2 cells.
Quantity
25 μg
Research Discipline
Signaling
Gene ID (Entrez)
NP_004422
Target Species
Human
Form
Purified
Applications
Flow Cytometry, Immunoprecipitation, Inhibition Assays
Clone
F2-27
Dilution
Inhibition Analysis: A representative lot inhibited cancer cell invasion in a three-dimensional spheroid formation assay. (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).Immunoprecipitation Analysis: A representative lot detected EphA2 in Immunoprecipitation applications (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).Flow Cytometry Analysis: A representative lot detected EphA2 in Flow Cytometry applications (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).
Gene Alias
Ephrin type-A receptor 2;EC: 2.7.10.1;Epithelial cell kinase;Tyrosine-protein kinase receptor ECK
Host Species
Mouse
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Clone F2-27 specifically detects Ephrin type -A receptor 2 in human.
Content And Storage
Stable for 1 year at 2-8°C from date of receipt.
Isotype
IgG1 κ
Related Products
Description
- Anti-EphA2, clone F2-27, Cat
- No
- MABS2026, is a mouse monoclonal antibody that detects Ephrin type-A receptor 2 and has been tested for use in Flow Cytometry, Immunoprecipitation, and Inhibition Assay
- Ephrin type-A receptor 2 (UniProt: P29317; also known as EC: 2.7.10.1, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK) is encoded by the EPHA2 (also known as ECK) gene (Gene ID: 1969) in human
- Ephrin type-A receptor 2 is a homodimeric single-pass type I membrane protein that is expressed in brain and glioma tissue and in glioma cell lines
- It is also highly expressed in tissues that contain a high proportion of epithelial cells
- Its levels are shown to be up-regulated by UV irradiation via a TP53-independent, MAPK-dependent mechanism
- Ephrin type-A receptor 2 serves as a receptor tyrosine kinase that binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells
- It is involved in the regulation of migration, integrin-mediated adhesion, and in proliferation and differentiation of cells
- Ephrin type-A receptor 2 is phosphorylated on tyrosine residues upon binding and activation by EFNA1
- Phosphorylation on Tyr 588 and Tyr 594 is reported to be essential for binding VAV2 and VAV3 while phosphorylation on Tyr 735 and Tyr 930 are required for binding PI3-kinase p85 subunit
- It is synthesized with a signal peptide (aa 1-23) that is cleaved off in the mature form
- The mature form has an extracellular domain (aa 24-537), a short transmembrane domain (aa 538-558), and a cytoplasmic domain (aa 559-976)
- Two isoforms of Ephrin type-A receptor 2 have been described that are produced by alternative splicing
- EphA2 is reported to be overexpressed in skin tumors murine models and its overexpression may represent a compensatory feedback mechanism during tumorigenesis
- (Ref.: Guo, H., et al
- (2006)
- Cancer Res
- 66(14); 7050-7058).
Compare Similar Items
Show Difference
Antigen: EphA2
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: EPHA2;ECK
Immunogen: KP-2, KP-3, SUIT-2, and MIAPaCa-2 cells.
Quantity: 25 μg
Research Discipline: Signaling
Gene ID (Entrez): NP_004422
Target Species: Human
Form: Purified
Applications: Flow Cytometry, Immunoprecipitation, Inhibition Assays
Clone: F2-27
Dilution: Inhibition Analysis: A representative lot inhibited cancer cell invasion in a three-dimensional spheroid formation assay. (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).Immunoprecipitation Analysis: A representative lot detected EphA2 in Immunoprecipitation applications (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).Flow Cytometry Analysis: A representative lot detected EphA2 in Flow Cytometry applications (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).
Gene Alias: Ephrin type-A receptor 2;EC: 2.7.10.1;Epithelial cell kinase;Tyrosine-protein kinase receptor ECK
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone F2-27 specifically detects Ephrin type -A receptor 2 in human.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
EphA2
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
EPHA2;ECK
Immunogen:
KP-2, KP-3, SUIT-2, and MIAPaCa-2 cells.
Quantity:
25 μg
Research Discipline:
Signaling
Gene ID (Entrez):
NP_004422
Target Species:
Human
Form:
Purified
Applications:
Flow Cytometry, Immunoprecipitation, Inhibition Assays
Clone:
F2-27
Dilution:
Inhibition Analysis: A representative lot inhibited cancer cell invasion in a three-dimensional spheroid formation assay. (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).Immunoprecipitation Analysis: A representative lot detected EphA2 in Immunoprecipitation applications (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).Flow Cytometry Analysis: A representative lot detected EphA2 in Flow Cytometry applications (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).
Gene Alias:
Ephrin type-A receptor 2;EC: 2.7.10.1;Epithelial cell kinase;Tyrosine-protein kinase receptor ECK
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone F2-27 specifically detects Ephrin type -A receptor 2 in human.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: SREBP-1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: SREBF1;BHLHD1;SREBP1
Immunogen: His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Quantity: 100 μg
Research Discipline: Signaling
Gene ID (Entrez): NP_001005291.1
Target Species: Hamster, Canine, Human
Form: Purified
Applications: Western Blot
Clone: 2A4
Dilution: Western Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Gene Alias: Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
SREBP-1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
SREBF1;BHLHD1;SREBP1
Immunogen:
His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Quantity:
100 μg
Research Discipline:
Signaling
Gene ID (Entrez):
NP_001005291.1
Target Species:
Hamster, Canine, Human
Form:
Purified
Applications:
Western Blot
Clone:
2A4
Dilution:
Western Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Gene Alias:
Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: SREBP-1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols: SREBF1;BHLHD1;SREBP1
Immunogen: His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Quantity: 25 μg
Research Discipline: Signaling
Gene ID (Entrez): NP_001005291.1
Target Species: Hamster, Canine, Human
Form: Purified
Applications: Western Blot
Clone: 2A4
Dilution: Western Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Gene Alias: Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species: Mouse
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
Isotype: IgG1 κ
Antigen:
SREBP-1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Symbols:
SREBF1;BHLHD1;SREBP1
Immunogen:
His-tagged recombinant fragment corresponding to 107 amino acids from the N-terminal half of human Sterol regulatory element-binding protein 1 (SREBP-1).
Quantity:
25 μg
Research Discipline:
Signaling
Gene ID (Entrez):
NP_001005291.1
Target Species:
Hamster, Canine, Human
Form:
Purified
Applications:
Western Blot
Clone:
2A4
Dilution:
Western Blotting Analysis: A representative lot detected SREBP-1 in Western Blotting applications (Toth, J.I., et. al. (2004). Mol Cell Biol. 24(18):8288-300; Key, C.C., et. al. (2017). Cell Rep. 19(10):2116-2129; Rosenfeld, J.M., et. al. (1998). J Biol Chem. 273(26):16112-21; Sato, R., et. al. (1994). J Biol Chem. 269(25):17267-73).
Gene Alias:
Sterol regulatory element-binding protein 1;Class D basic helix-loop-helix protein 1;bHLHd1;Sterol regulatory element-binding transcription factor 1
Host Species:
Mouse
Purification Method:
Protein G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 2A4 is a mouse monoclonal antibody that detects Sterol regulatory element-binding protein 1. It targets an epitope within 107 amino acids from the N-terminal half.
Content And Storage:
Stable for 1 year at 2-8°C from date of receipt.
Isotype:
IgG1 κ
Antigen: Factor XII
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: __
Gene Symbols: F12
Immunogen: A mixture of purified human Factor XII and Factor XIIa
Quantity: 100 μg
Research Discipline: Signaling
Gene ID (Entrez): NP_000496
Target Species: Baboon, Human
Form: Purified
Applications: ELISA, Inhibition Assays, Western Blot
Clone: 15H8
Dilution: __
Gene Alias: __
Host Species: Mouse
Purification Method: Protein G Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Clone 15H8 is a mouse monoclonal antibody that specifically detects Coagulation factor XII.
Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype: IgG2a κ
Antigen:
Factor XII
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
__
Gene Symbols:
F12
Immunogen:
A mixture of purified human Factor XII and Factor XIIa
Quantity:
100 μg
Research Discipline:
Signaling
Gene ID (Entrez):
NP_000496
Target Species:
Baboon, Human
Form:
Purified
Applications:
ELISA, Inhibition Assays, Western Blot
Clone:
15H8
Dilution:
__
Gene Alias:
__
Host Species:
Mouse
Purification Method:
Protein G Purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Clone 15H8 is a mouse monoclonal antibody that specifically detects Coagulation factor XII.
Content And Storage:
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Isotype:
IgG2a κ