Bcl-2 Antibody (100/D5), Novus Biologicals™
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NBP21520002-Each-of-1 | In Stock | ₹ 55,015.08 |
NBP21520002 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 55,015.08
GST (18%): ₹ 9,902.714
Total Price: ₹ 64,917.794
Antigen
Bcl-2
Classification
Monoclonal
Concentration
0.2mg/mL
Dilution
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Gene Accession No.
P10415
Gene Symbols
BCL2
Immunogen
The synthetic peptide corresponding to amino acids range from 41-64 (GAAPAPGIFSSQPG-Cys), of human Bcl-2 was used as immunogen to generate the antibody; GenBank no. NP_000648.2 (Pezzella et al, 1990).
Purification Method
Protein G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
This antibody recognizes a protein of 25-26kDa, identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Target Species
Human, Mouse (Negative), Rat (Negative)
Form
Purified
Applications
Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Clone
100/D5
Conjugate
Unconjugated
Formulation
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias
apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
Host Species
Mouse
Molecular Weight of Antigen
25 kDa
Quantity
0.2 mg
Research Discipline
Apoptosis, Autophagy, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Tumor Suppressors
Gene ID (Entrez)
596
Reconstitution
Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
Content And Storage
Store at 4C.
Isotype
IgG1 κ
Related Products
Description
- Bcl-2 Monoclonal specifically detects Bcl-2 in Human, Mouse (Negative), Rat (Negative) samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular).
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Antigen: Bcl-2
Classification: Monoclonal
Concentration: 0.2mg/mL
Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Gene Accession No.: P10415
Gene Symbols: BCL2
Immunogen: The synthetic peptide corresponding to amino acids range from 41-64 (GAAPAPGIFSSQPG-Cys), of human Bcl-2 was used as immunogen to generate the antibody; GenBank no. NP_000648.2 (Pezzella et al, 1990).
Purification Method: Protein G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This antibody recognizes a protein of 25-26kDa, identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Target Species: Human, Mouse (Negative), Rat (Negative)
Form: Purified
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Clone: 100/D5
Conjugate: Unconjugated
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, Bcl-2
Host Species: Mouse
Molecular Weight of Antigen: 25 kDa
Quantity: 0.2 mg
Research Discipline: Apoptosis, Autophagy, Cancer, Cellular Markers, Mitochondrial Mediated Pathway, Mitophagy, Tumor Suppressors
Gene ID (Entrez): 596
Reconstitution: Outer mitochondrial membranes and endoplasmic reticulum as well as nuclear membranes.
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Antigen: CD31/PECAM-1
Classification: Monoclonal
Concentration: 0.2mg/mL
Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Gene Accession No.: P16284
Gene Symbols: PECAM1
Immunogen: Human recombinant CD31 protein
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Target Species: Human
Form: Purified
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Clone: C31.3
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: adhesion molecule, CD31, CD31 antigen, CD31/EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA', PECA1, PECAM-1, PECAM-1, CD31/EndoCAM, platelet endothelial cell adhesion molecule, platelet/endothelial cell adhesion molecule
Host Species: Mouse
Molecular Weight of Antigen: __
Quantity: 0.1 mg
Research Discipline: Angiogenesis, Cancer, Cellular Markers, Cytoskeleton Markers, Embryonic Stem Cell Markers, Endothelial Cell Markers, Extracellular Matrix, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Signal Transduction, Stem Cell Markers
Gene ID (Entrez): 5175
Reconstitution: __
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Antigen: ABCF3
Classification: Polyclonal
Concentration: 0.74mg/mL
Dilution: Western Blot 1:500-1:3000, Immunohistochemistry 10 - 1:500, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunohistochemistry-Paraffin 1:100-1:1000
Gene Accession No.: Q9NUQ8
Gene Symbols: ABCF3
Immunogen: Recombinant protein encompassing a sequence within the center region of human ABCF3. The exact sequence is proprietary.
Purification Method: Affinity Purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Human. Expected to cross react based on sequence identity: Mouse (95%), Rat (93%), Bovine (96%).
Target Species: Human
Form: __
Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone: __
Conjugate: Unconjugated
Formulation: 0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol with 0.01% Thimerosal
Gene Alias: ATP-binding cassette sub-family F member 3, ATP-binding cassette, sub-family F (GCN20), member 3, EC 3.6.3, EC 3.6.3.17, EST201864, FLJ11198
Host Species: Rabbit
Molecular Weight of Antigen: 80 kDa
Quantity: 0.1 mL
Research Discipline: __
Gene ID (Entrez): 55324
Reconstitution: __
Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Isotype: IgG
