NBP229409

Cytokeratin 18 Antibody (DA7), Novus Biologicals™

Manufacturer: Novus Biologicals

Select a Size

Pack Size SKU Availability Price
Each of 1 NBP229409-Each-of-1 In Stock ₹ 44,833.44

NBP229409 - Each of 1

₹ 44,833.44

In Stock

Quantity

1

Base Price: ₹ 44,833.44

GST (18%): ₹ 8,070.019

Total Price: ₹ 52,903.459

Antigen

Cytokeratin 18

Classification

Monoclonal

Concentration

0.2 mg/ml

Dilution

Western Blot 1-3 μg/mL, Simple Western 10 μg/mL, Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1-2 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1.0 μg/mL

Gene Alias

Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18

Host Species

Mouse

Purification Method

Protein A or G purified

Regulatory Status

RUO

Primary or Secondary

Primary

Test Specificity

This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Content And Storage

Store at 4C.

Isotype

IgG1 κ

Applications

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone

DA7

Conjugate

Unconjugated

Gene Accession No.

P05783

Gene Symbols

KRT18

Immunogen

Human breast cancer PMC 42 cells were used as immunogen to generate the Cytokeratin 18 (CK18) antibody.

Quantity

0.1 mg

Research Discipline

Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Phospho Specific

Gene ID (Entrez)

3875

Target Species

Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative)

Form

Purified

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Description

  • Cytokeratin 18 Monoclonal specifically detects Cytokeratin 18 in Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative), Sheep (Negative) samples
  • It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Compare Similar Items

Show Difference

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Novus Biologicals

NBP229409

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Antigen:
Cytokeratin 18

Classification:
Monoclonal

Concentration:
0.2 mg/ml

Dilution:
Western Blot 1-3 μg/mL, Simple Western 10 μg/mL, Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1-2 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 0.5-1.0 μg/mL

Gene Alias:
Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Content And Storage:
Store at 4C.

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Clone:
DA7

Conjugate:
Unconjugated

Gene Accession No.:
P05783

Gene Symbols:
KRT18

Immunogen:
Human breast cancer PMC 42 cells were used as immunogen to generate the Cytokeratin 18 (CK18) antibody.

Quantity:
0.1 mg

Research Discipline:
Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers, Phospho Specific

Gene ID (Entrez):
3875

Target Species:
Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative)

Form:
Purified

Img

Novus Biologicals

NBP229411

--


Antigen:
Cytokeratin, LMW

Classification:
Monoclonal

Concentration:
0.2 mg/ml

Dilution:
Western Blot 0.5-1 μg/mL, Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mL

Gene Alias:
Cytokeratin LMW, cytokeratin-1B, keratin 77, keratin, type II cytoskeletal 1b, KRT77, type-II keratin Kb39

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Primary or Secondary:
Primary

Test Specificity:
This monoclonal antibody recognizes the 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI, 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Content And Storage:
Store at 4C.

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone:
AE-1

Conjugate:
Unconjugated

Gene Accession No.:
P04264

Gene Symbols:
KRT77

Immunogen:
Human epidermal keratin (Uniprot: Q7Z794)

Quantity:
0.1 mg

Research Discipline:
__

Gene ID (Entrez):
374454

Target Species:
Human, Mouse, Rat, Bovine, Canine, Chicken, Other, Primate, Rabbit

Form:
Purified

Img

Novus Biologicals

NBP22941549

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Antigen:
TCRalpha/beta

Classification:
Monoclonal

Concentration:
__

Dilution:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Gene Alias:
C), D, FLJ22602, J, MGC117436, MGC22624, MGC23964, MGC71411, T cell receptor alpha locus, T-cell antigen receptor, alpha polypeptide, T-cell receptor, alpha (V, TCRA, TCRD, TRA

Host Species:
Mouse

Purification Method:
Protein A purified

Regulatory Status:
__

Primary or Secondary:
Primary

Test Specificity:
The mouse monoclonal R73 recognizes TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.

Content And Storage:
Store at 4°C in the dark.

Isotype:
IgG1 κ

Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)

Clone:
R73

Conjugate:
Janelia Fluor 549

Gene Accession No.:
__

Gene Symbols:
TRA

Immunogen:
Rat T blasts and erythrocytes

Quantity:
0.1 mL

Research Discipline:
Adaptive Immunity, Cell Biology, Diabetes Research, Immunology, Protein Kinase, Signal Transduction, Tyrosine Kinases

Gene ID (Entrez):
6955

Target Species:
Primate, Rat

Form:
Purified

Img

Novus Biologicals

NBP22941646

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Antigen:
TCRalpha/beta

Classification:
Monoclonal

Concentration:
__

Dilution:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen

Gene Alias:
C), D, FLJ22602, J, MGC117436, MGC22624, MGC23964, MGC71411, T cell receptor alpha locus, T-cell antigen receptor, alpha polypeptide, T-cell receptor, alpha (V, TCRA, TCRD, TRA

Host Species:
Mouse

Purification Method:
Protein A purified

Regulatory Status:
__

Primary or Secondary:
Primary

Test Specificity:
The mouse monoclonal R73 recognizes TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.

Content And Storage:
Store at 4°C in the dark.

Isotype:
IgG1 κ

Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)

Clone:
R73

Conjugate:
Janelia Fluor 646

Gene Accession No.:
__

Gene Symbols:
TRA

Immunogen:
Rat T blasts and erythrocytes

Quantity:
0.1 mL

Research Discipline:
Adaptive Immunity, Cell Biology, Diabetes Research, Immunology, Protein Kinase, Signal Transduction, Tyrosine Kinases

Gene ID (Entrez):
6955

Target Species:
Primate, Rat

Form:
Purified