NBP229402

Phospho-Tyrosine Antibody (PY20), Novus Biologicals™

Manufacturer: Novus Biologicals

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Pack Size SKU Availability Price
Each of 1 NBP229402-Each-of-1 In Stock ₹ 44,833.44

NBP229402 - Each of 1

₹ 44,833.44

In Stock

Quantity

1

Base Price: ₹ 44,833.44

GST (18%): ₹ 8,070.019

Total Price: ₹ 52,903.459

Antigen

Phospho-Tyrosine

Classification

Monoclonal

Concentration

0.2 mg/ml

Dilution

Western Blot 0.5-1 μg/mL, Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1-2 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 1-2 μg/mL, Immunohistochemistry-Frozen 1-2 μg/mL

Host Species

Mouse

Purification Method

Protein A or G purified

Regulatory Status

RUO

Test Specificity

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This monoclonal antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Content And Storage

Store at 4C.

Isotype

IgG2b

Applications

Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone

PY20

Conjugate

Unconjugated

Gene Alias

2 amino 3(4 hydroxyphenyl) propanoic acid, 4 hydroxyphenylalanine, phosphotyrosine, pTyrosine, Tyrosine

Immunogen

Phosphotyrosine was used as the immunogen for the Phosphotyrosine PY20 antibody.

Quantity

0.1 mg

Primary or Secondary

Primary

Target Species

All species

Form

Purified

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Description

  • Phospho-Tyrosine Monoclonal specifically detects Phospho-Tyrosine in All Species samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin.

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Novus Biologicals

NBP229402

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Antigen:
Phospho-Tyrosine

Classification:
Monoclonal

Concentration:
0.2 mg/ml

Dilution:
Western Blot 0.5-1 μg/mL, Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunoprecipitation 1-2 μg/500 μg protein lysate, Immunohistochemistry-Paraffin 1-2 μg/mL, Immunohistochemistry-Frozen 1-2 μg/mL

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Test Specificity:
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This monoclonal antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.

Content And Storage:
Store at 4C.

Isotype:
IgG2b

Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone:
PY20

Conjugate:
Unconjugated

Gene Alias:
2 amino 3(4 hydroxyphenyl) propanoic acid, 4 hydroxyphenylalanine, phosphotyrosine, pTyrosine, Tyrosine

Immunogen:
Phosphotyrosine was used as the immunogen for the Phosphotyrosine PY20 antibody.

Quantity:
0.1 mg

Primary or Secondary:
Primary

Target Species:
All species

Form:
Purified

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NBP229403

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Antigen:
S100A1

Classification:
Monoclonal

Concentration:
0.2 mg/ml

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Test Specificity:
S100 belongs to the family of calcium binding proteins. S100A and S100B proteins are two members of the S100 family. S100A is composed of an alpha and a beta chain whereas S100B is composed of two beta chains. This antibody is specific against an epitope located on the beta-chain (i.e. in S-100A and S-100B) but not on the alpha-chain of S-100 (i.e. in S-100A and S100A0). This antibody can be used to localize S-100A and S-100B in various tissue sections. S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well-differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell-derived tumors express S-100 protein. Almost all malignant melanomas and cases of

Content And Storage:
Store at 4C.

Isotype:
IgG2a κ

Applications:
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Clone:
4C4.9

Conjugate:
Unconjugated

Gene Alias:
protein S100-A1, S100 alpha, S100 calcium binding protein A1, S100 calcium-binding protein A1S100, S-100 protein alpha chain, S-100 protein subunit alpha, S100 protein, alpha polypeptide, S100A, S100-alpha

Immunogen:
Purified bovine brain S100B protein (Uniprot: P04271)

Quantity:
0.1 mg

Primary or Secondary:
Primary

Target Species:
Human, Mouse, Rat, Bovine

Form:
Purified

Img

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NBP229404

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Antigen:
Cytokeratin, HMW

Classification:
Monoclonal

Concentration:
0.2 mg/ml

Dilution:
Western Blot 0.5-1 μg/mL, Flow Cytometry 0.5-1 μg/million cells, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mL

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Test Specificity:
This monoclonal antibody recognizes basic (Type II or HMW) cytokeratins, which include 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50', 48, 46, 45, and 40kDa. monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer.

Content And Storage:
Store at 4C.

Isotype:
IgG1 κ

Applications:
Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Clone:
AE-3

Conjugate:
Unconjugated

Gene Alias:
CK-2P, cytokeratin 2, Cytokeratin-2P, HUMCYT2A, K2P, K76, keratin 76, keratin, type II cytoskeletal 2 oral, Keratin-76, KRT2Bcytokeratin-2P, KRT2Pkeratin 2p, KRT76, Type-II keratin Kb9

Immunogen:
Human epidermal keratin (Uniprot: Q01546)

Quantity:
0.1 mg

Primary or Secondary:
Primary

Target Species:
Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit

Form:
Purified

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NBP229407

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Antigen:
PMEL17/SILV

Classification:
Monoclonal

Concentration:
0.2 mg/ml

Dilution:
Immunohistochemistry, Immunohistochemistry-Paraffin 1-2 ug/ml, Protein Array, Flow (Intracellular)

Host Species:
Mouse

Purification Method:
Protein A or G purified

Regulatory Status:
RUO

Test Specificity:
By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Content And Storage:
Store at 4C.

Isotype:
IgG2b κ

Applications:
Immunohistochemistry, Immunohistochemistry (Paraffin), Peptide Array, Flow Cytometry, Immunohistochemistry (Frozen)

Clone:
NKI-beteb

Conjugate:
Unconjugated

Gene Alias:
D12S53EP1, gp100, ME20, ME20-M, melanocyte protein mel 17, Melanocyte protein Pmel 17, Melanocytes lineage-specific antigen GP100, Melanoma-associated ME20 antigen, melanosomal matrix protein17, PMEL17P100, premelanosome proteinME20M, SI, SIL, silver (mouse homolog) like, silver homolog (mouse), Silver locus protein homolog, silver, mouse, homolog of, SILVPmel17

Immunogen:
Lymph node pigmented melanoma metastases extract was used as the immunogen for the gp100 NKI-beteb antibody

Quantity:
0.1 mg

Primary or Secondary:
Primary

Target Species:
Human, Equine

Form:
Purified