Cytokeratin, pan Antibody (AE-1/AE-3), Novus Biologicals™
Manufacturer: Novus Biologicals
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NBP229429-Each-of-1 | In Stock | ₹ 45,860.16 |
NBP229429 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 45,860.16
GST (18%): ₹ 8,254.829
Total Price: ₹ 54,114.989
Antigen
pan Cytokeratin
Classification
Monoclonal
Concentration
0.2 mg/ml
Dilution
Western Blot 1 - 2 ug/mL, Flow Cytometry 1 - 2 ug/ million cells, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1 - 2 ug/mL, Immunohistochemistry-Paraffin 0.25 - 1.0 ug/mL, Immunohistochemistry-Frozen 0.25 - 1.0 ug/mL, Flow (Intracellular), CyTOF-reported, Single Cell Western 1:10, Dual RNAscope ISH-IHC
Gene Symbols
KRT1
Immunogen
This Cytokeratin, pan Antibody (AE-1/AE-3) was developed against total keratin isolated from human epidermal callus was used as immunogen to generate the pan cytokeratin antibodies AE1 + AE3 (Woodcock-Mitchell, 1982).
Quantity
0.1 mg
Research Discipline
Apoptosis, Cancer, Cell Biology, Cellular Markers, Signal Transduction
Gene ID (Entrez)
3848
Target Species
Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit, Reptile, Zebrafish
Form
Purified
Applications
Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), CyTOF, Western Blot, In Situ Hybridization (ISH)
Clone
AE-1/AE-3
Conjugate
Unconjugated
Gene Accession No.
Q7Z794, Q01546
Host Species
Mouse
Purification Method
Protein A or G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues
Content And Storage
Store at 4C.
Isotype
IgG1 κ
Related Products
Description
- Cytokeratin, pan Monoclonal specifically detects Cytokeratin, pan in Human, Mouse, Rat, Bovine, Canine, Chicken, Monkey, Rabbit, Reptile, Zebrafish samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow (Intracellular), CyTOF-reported, Single Cell Western, Dual RNAscope ISH-IHC.
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Antigen: pan Cytokeratin
Classification: Monoclonal
Concentration: 0.2 mg/ml
Dilution: Western Blot 1 - 2 ug/mL, Flow Cytometry 1 - 2 ug/ million cells, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1 - 2 ug/mL, Immunohistochemistry-Paraffin 0.25 - 1.0 ug/mL, Immunohistochemistry-Frozen 0.25 - 1.0 ug/mL, Flow (Intracellular), CyTOF-reported, Single Cell Western 1:10, Dual RNAscope ISH-IHC
Gene Symbols: KRT1
Immunogen: This Cytokeratin, pan Antibody (AE-1/AE-3) was developed against total keratin isolated from human epidermal callus was used as immunogen to generate the pan cytokeratin antibodies AE1 + AE3 (Woodcock-Mitchell, 1982).
Quantity: 0.1 mg
Research Discipline: Apoptosis, Cancer, Cell Biology, Cellular Markers, Signal Transduction
Gene ID (Entrez): 3848
Target Species: Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit, Reptile, Zebrafish
Form: Purified
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), CyTOF, Western Blot, In Situ Hybridization (ISH)
Clone: AE-1/AE-3
Conjugate: Unconjugated
Gene Accession No.: Q7Z794, Q01546
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which 67kDa (CK1); 64kDa (CK3); 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 50kDa (CK14); 50kDa (CK15); 48kDa (CK16); 40kDa (CK19). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Antigen: NKX2.2
Classification: Monoclonal
Concentration: 0.2 mg/mL
Dilution: Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Gene Symbols: NKX2-2
Immunogen: Human full-length recombinant NKX2.2 protein (Uniprot: O95096)
Quantity: 0.1 mg
Research Discipline: __
Gene ID (Entrez): 4821
Target Species: Human, Mouse, Rat, Chicken
Form: Purified
Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Clone: NX2/294
Conjugate: Unconjugated
Gene Accession No.: O95096
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.
Content And Storage: Store at 4C.
Isotype: IgG2b κ
Antigen: Vimentin
Classification: Monoclonal
Concentration: 0.2 mg/ml
Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, ELISA 1-5ug/ml for coating, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml, Knockout Validated 0.7 ug/ml
Gene Symbols: VIM
Immunogen: Recombinant full-length human vimentin protein (Uniprot: P08670)
Quantity: 0.1 mg
Research Discipline: Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Phospho Specific, Signal Transduction, Stem Cell Markers, Stem Cells
Gene ID (Entrez): 7431
Target Species: Human, Porcine, Bovine, Canine, Chicken, Feline, Goat, Mouse (Negative), Rat (Negative)
Form: Purified
Applications: Western Blot, Flow Cytometry, ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Clone: VM452
Conjugate: Unconjugated
Gene Accession No.: P08670
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: This monoclonal antibody reacts with a 58kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of Vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-Vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-Vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain Vimentin, e.g. hepatoma and seminoma. Anti-Vimentin is also useful as a tissue process control reagent.
Content And Storage: Store at 4C.
Isotype: IgG1 κ
