NBP247859B
CD53 Antibody (161-2) - Azide and BSA Free, Novus Biologicals™
Manufacturer: Fischer Scientific
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Antigen
CD53
Classification
Monoclonal
Concentration
1.0 mg/mL
Dilution
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias
antigen MOX44 identified by monoclonal MRC-OX44, CD53 antigentetraspanin-25, CD53 glycoprotein, CD53 molecule, CD53 tetraspan antigen, cell surface antigen, Cell surface glycoprotein CD53, MOX44transmembrane glycoprotein, Tetraspanin-25, tspan-25, TSPAN25leukocyte surface antigen CD53
Host Species
Mouse
Purification Method
Protein A or G purified
Regulatory Status
RUO
Primary or Secondary
Primary
Test Specificity
Recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype
IgG2a κ
Applications
Flow Cytometry, Immunofluorescence, CyTOF
Clone
161-2
Conjugate
Unconjugated
Formulation
PBS with No Preservative
Gene Symbols
CD53
Immunogen
Stimulated human leukocytes
Quantity
0.2 mg
Research Discipline
Immunology
Gene ID (Entrez)
963
Target Species
Human, Baboon (Negative), Equine (Negative)
Form
Purified
Related Products
Description
- CD53 Monoclonal specifically detects CD53 in Human, Baboon (Negative), Equine (Negative) samples
- It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Functional, Immunofluorescence, CyTOF-ready.
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Antigen: CD53
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias: antigen MOX44 identified by monoclonal MRC-OX44, CD53 antigentetraspanin-25, CD53 glycoprotein, CD53 molecule, CD53 tetraspan antigen, cell surface antigen, Cell surface glycoprotein CD53, MOX44transmembrane glycoprotein, Tetraspanin-25, tspan-25, TSPAN25leukocyte surface antigen CD53
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG2a κ
Applications: Flow Cytometry, Immunofluorescence, CyTOF
Clone: 161-2
Conjugate: Unconjugated
Formulation: PBS with No Preservative
Gene Symbols: CD53
Immunogen: Stimulated human leukocytes
Quantity: 0.2 mg
Research Discipline: Immunology
Gene ID (Entrez): 963
Target Species: Human, Baboon (Negative), Equine (Negative)
Form: Purified
Antigen:
CD53
Classification:
Monoclonal
Concentration:
1.0 mg/mL
Dilution:
Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias:
antigen MOX44 identified by monoclonal MRC-OX44, CD53 antigentetraspanin-25, CD53 glycoprotein, CD53 molecule, CD53 tetraspan antigen, cell surface antigen, Cell surface glycoprotein CD53, MOX44transmembrane glycoprotein, Tetraspanin-25, tspan-25, TSPAN25leukocyte surface antigen CD53
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes a protein of 33-55kDa, identified as CD53 (Workshop VI; Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG2a κ
Applications:
Flow Cytometry, Immunofluorescence, CyTOF
Clone:
161-2
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
CD53
Immunogen:
Stimulated human leukocytes
Quantity:
0.2 mg
Research Discipline:
Immunology
Gene ID (Entrez):
963
Target Species:
Human, Baboon (Negative), Equine (Negative)
Form:
Purified
Antigen: Nucleolin
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias: C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1 κ
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone: 364-5
Conjugate: Unconjugated
Formulation: PBS with No Preservative
Gene Symbols: NCL
Immunogen: Lysate of SU-DHL-1 Nuclei
Quantity: 0.1 mg
Research Discipline: Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Gene ID (Entrez): 4691
Target Species: Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Form: Purified
Antigen:
Nucleolin
Classification:
Monoclonal
Concentration:
1.0 mg/mL
Dilution:
Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG1 κ
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone:
364-5
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
NCL
Immunogen:
Lysate of SU-DHL-1 Nuclei
Quantity:
0.1 mg
Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Gene ID (Entrez):
4691
Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Form:
Purified
Antigen: Nucleolin
Classification: Monoclonal
Concentration: 1.0 mg/mL
Dilution: Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias: C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: RUO
Primary or Secondary: Primary
Test Specificity: Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype: IgG1 κ
Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone: 364-5
Conjugate: Unconjugated
Formulation: PBS with No Preservative
Gene Symbols: NCL
Immunogen: Lysate of SU-DHL-1 Nuclei
Quantity: 0.2 mg
Research Discipline: Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Gene ID (Entrez): 4691
Target Species: Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Form: Purified
Antigen:
Nucleolin
Classification:
Monoclonal
Concentration:
1.0 mg/mL
Dilution:
Western Blot : 0.5 - 1.0 ug/ml, Flow Cytometry : 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin : 0.25 - 0.5 ug/ml, Immunofluorescence : 0.5 - 1.0 ug/ml, CyTOF-ready
Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Primary or Secondary:
Primary
Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Isotype:
IgG1 κ
Applications:
Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Clone:
364-5
Conjugate:
Unconjugated
Formulation:
PBS with No Preservative
Gene Symbols:
NCL
Immunogen:
Lysate of SU-DHL-1 Nuclei
Quantity:
0.2 mg
Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Stem Cell Markers
Gene ID (Entrez):
4691
Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Form:
Purified
Antigen: Nucleolin
Classification: Monoclonal
Concentration: __
Dilution: Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence
Gene Alias: C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Host Species: Mouse
Purification Method: Protein A or G purified
Regulatory Status: __
Primary or Secondary: Primary
Test Specificity: Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Content And Storage: Store at 4C in the dark.
Isotype: IgG1 κ
Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone: 364-5 + NCL/902
Conjugate: FITC
Formulation: PBS with 0.05% Sodium Azide
Gene Symbols: NCL
Immunogen: Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902) (Uniprot: P19338 )
Quantity: 0.1 mL
Research Discipline: Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Phospho Specific, Stem Cell Markers
Gene ID (Entrez): 4691
Target Species: Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Form: Purified
Antigen:
Nucleolin
Classification:
Monoclonal
Concentration:
__
Dilution:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence
Gene Alias:
C23, FLJ45706, FLJ59041, nucleolin, Protein C23
Host Species:
Mouse
Purification Method:
Protein A or G purified
Regulatory Status:
__
Primary or Secondary:
Primary
Test Specificity:
Recognizes a protein of ∼76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. This monoclonal antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Content And Storage:
Store at 4C in the dark.
Isotype:
IgG1 κ
Applications:
Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone:
364-5 + NCL/902
Conjugate:
FITC
Formulation:
PBS with 0.05% Sodium Azide
Gene Symbols:
NCL
Immunogen:
Lysate of SU-DHL-1 Nuclei (364-5); Recombinant human NCL protein (NCL/902) (Uniprot: P19338 )
Quantity:
0.1 mL
Research Discipline:
Cell Cycle and Replication, Chromatin Research, Core ESC Like Genes, DNA Repair, DNA replication Transcription Translation and Splicing, Phospho Specific, Stem Cell Markers
Gene ID (Entrez):
4691
Target Species:
Human, Bovine (Negative), Mouse (Negative), Rat (Negative)
Form:
Purified