NBP248221
EMI1 Antibody (EMI1/1176) - IHC-Prediluted, Novus Biologicals™
Manufacturer: Novus Biologicals
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NBP248221-Each-of-1 | In Stock | ₹ 43,336.14 |
NBP248221 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 43,336.14
GST (18%): ₹ 7,800.505
Total Price: ₹ 51,136.645
Antigen
EMI1
Classification
Monoclonal
Conjugate
Unconjugated
Formulation
1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols
FBXO5
Immunogen
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein (Uniprot: Q9UKT4)
Purification Method
Protein A or G purified
Regulatory Status
RUO
Gene ID (Entrez)
26271
Target Species
Human
Form
Purified
Applications
Immunohistochemistry (Paraffin)
Clone
EMI1/1176
Dilution
Immunohistochemistry-Paraffin
Gene Alias
EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31
Host Species
Mouse
Molecular Weight of Antigen
56 kDa
Quantity
7 mL
Primary or Secondary
Primary
Test Specificity
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Content And Storage
Store at 4C.
Isotype
IgG2a κ
Related Products
Description
- EMI1 Monoclonal specifically detects EMI1 in Human samples
- It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.
Compare Similar Items
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Antigen: EMI1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols: FBXO5
Immunogen: Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein (Uniprot: Q9UKT4)
Purification Method: Protein A or G purified
Regulatory Status: RUO
Gene ID (Entrez): 26271
Target Species: Human
Form: Purified
Applications: Immunohistochemistry (Paraffin)
Clone: EMI1/1176
Dilution: Immunohistochemistry-Paraffin
Gene Alias: EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31
Host Species: Mouse
Molecular Weight of Antigen: 56 kDa
Quantity: 7 mL
Primary or Secondary: Primary
Test Specificity: It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Content And Storage: Store at 4C.
Isotype: IgG2a κ
Antigen:
EMI1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols:
FBXO5
Immunogen:
Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein (Uniprot: Q9UKT4)
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Gene ID (Entrez):
26271
Target Species:
Human
Form:
Purified
Applications:
Immunohistochemistry (Paraffin)
Clone:
EMI1/1176
Dilution:
Immunohistochemistry-Paraffin
Gene Alias:
EMI1Early mitotic inhibitor 1, F-box only protein 5, F-box protein 5, FBX5F-box protein Fbx5, Fbxo31
Host Species:
Mouse
Molecular Weight of Antigen:
56 kDa
Quantity:
7 mL
Primary or Secondary:
Primary
Test Specificity:
It recognizes a 56kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.
Content And Storage:
Store at 4C.
Isotype:
IgG2a κ
Antigen: EMMPRIN/CD147
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols: BSG
Immunogen: Recombinant human EMMPRIN/CD147 protein (Uniprot: P35613)
Purification Method: Protein A or G purified
Regulatory Status: RUO
Gene ID (Entrez): 682
Target Species: Human
Form: Purified
Applications: Immunohistochemistry (Paraffin)
Clone: BSG/963
Dilution: Immunohistochemistry-Paraffin
Gene Alias: 5F7, basigin, basigin (Ok blood group), CD147, CD147 antigen, Collagenase stimulatory factor, EMMPRINTCSF, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, M6, OK, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor
Host Species: Mouse
Molecular Weight of Antigen: __
Quantity: 7 mL
Primary or Secondary: Primary
Test Specificity: This monoclonal antibody recognizes extracellular epitope of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Antigen:
EMMPRIN/CD147
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols:
BSG
Immunogen:
Recombinant human EMMPRIN/CD147 protein (Uniprot: P35613)
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Gene ID (Entrez):
682
Target Species:
Human
Form:
Purified
Applications:
Immunohistochemistry (Paraffin)
Clone:
BSG/963
Dilution:
Immunohistochemistry-Paraffin
Gene Alias:
5F7, basigin, basigin (Ok blood group), CD147, CD147 antigen, Collagenase stimulatory factor, EMMPRINTCSF, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, M6, OK, OK blood group antigen, Tumor cell-derived collagenase stimulatory factor
Host Species:
Mouse
Molecular Weight of Antigen:
__
Quantity:
7 mL
Primary or Secondary:
Primary
Test Specificity:
This monoclonal antibody recognizes extracellular epitope of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.
Content And Storage:
Store at 4C.
Isotype:
IgG1 κ
Antigen: WT1
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols: WT1
Immunogen: Recombinant full length human WT1 protein (WT1/857); Recombinant fragment aa1-181 of human WT1 (6F-H2) (Uniprot: P19544)
Purification Method: Protein A or G purified
Regulatory Status: RUO
Gene ID (Entrez): 7490
Target Species: Human, Mouse, Rat
Form: Purified
Applications: Immunohistochemistry (Paraffin)
Clone: WT1/857 + 6F-H2
Dilution: Immunohistochemistry-Paraffin
Gene Alias: AWT1, NPHS4GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT-2, WT33
Host Species: Mouse
Molecular Weight of Antigen: __
Quantity: 7 mL
Primary or Secondary: Primary
Test Specificity: Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Content And Storage: Store at 4C.
Isotype: IgG1 κ
Antigen:
WT1
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols:
WT1
Immunogen:
Recombinant full length human WT1 protein (WT1/857); Recombinant fragment aa1-181 of human WT1 (6F-H2) (Uniprot: P19544)
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Gene ID (Entrez):
7490
Target Species:
Human, Mouse, Rat
Form:
Purified
Applications:
Immunohistochemistry (Paraffin)
Clone:
WT1/857 + 6F-H2
Dilution:
Immunohistochemistry-Paraffin
Gene Alias:
AWT1, NPHS4GUD, WAGR, Wilms tumor 1, Wilms tumor protein, WIT-2, WT33
Host Species:
Mouse
Molecular Weight of Antigen:
__
Quantity:
7 mL
Primary or Secondary:
Primary
Test Specificity:
Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Content And Storage:
Store at 4C.
Isotype:
IgG1 κ
Antigen: ZAP70
Classification: Monoclonal
Conjugate: Unconjugated
Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols: ZAP70
Immunogen: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70 (Uniprot: P43403)
Purification Method: Protein A or G purified
Regulatory Status: RUO
Gene ID (Entrez): 7535
Target Species: Human
Form: Purified
Applications: Immunohistochemistry, Immunohistochemistry (Paraffin)
Clone: 2F3.2
Dilution: Immunohistochemistry, Immunohistochemistry-Paraffin
Gene Alias: 70 kDa zeta-associated protein, EC 2.7.10, EC 2.7.10.2, SRKFLJ17679, STDFLJ17670, Syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, TZK, ZAP-70, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD
Host Species: Mouse
Molecular Weight of Antigen: 70 kDa
Quantity: 7 mL
Primary or Secondary: Primary
Test Specificity: ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Content And Storage: Store at 4C.
Isotype: IgG2a κ
Antigen:
ZAP70
Classification:
Monoclonal
Conjugate:
Unconjugated
Formulation:
1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Symbols:
ZAP70
Immunogen:
Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70 (Uniprot: P43403)
Purification Method:
Protein A or G purified
Regulatory Status:
RUO
Gene ID (Entrez):
7535
Target Species:
Human
Form:
Purified
Applications:
Immunohistochemistry, Immunohistochemistry (Paraffin)
Clone:
2F3.2
Dilution:
Immunohistochemistry, Immunohistochemistry-Paraffin
Gene Alias:
70 kDa zeta-associated protein, EC 2.7.10, EC 2.7.10.2, SRKFLJ17679, STDFLJ17670, Syk-related tyrosine kinase, tyrosine-protein kinase ZAP-70, TZK, ZAP-70, zeta-chain (TCR) associated protein kinase (70 kD), zeta-chain (TCR) associated protein kinase 70kDa, zeta-chain associated protein kinase, 70kD
Host Species:
Mouse
Molecular Weight of Antigen:
70 kDa
Quantity:
7 mL
Primary or Secondary:
Primary
Test Specificity:
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Content And Storage:
Store at 4C.
Isotype:
IgG2a κ