NBP275766
PU.1/Spi-1 Antibody (PU1/2146) - Azide and BSA Free, Novus Biologicals™
Manufacturer: Novus Biologicals
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | NBP275766-Each-of-1 | In Stock | ₹ 55,271.76 |
NBP275766 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 55,271.76
GST (18%): ₹ 9,948.917
Total Price: ₹ 65,220.677
Antigen
PU.1/Spi-1
Classification
Monoclonal
Concentration
0.2 mg/ml
Dilution
Western Blot 0.5-1 ug/ml, Immunohistochemistry-Paraffin 1-2 ug/ml
Gene Accession No.
P17947
Gene Symbols
SPI1
Immunogen
Recombinant fragment (around aa 16-170) of human PU.1/Spi-1 protein (Exact sequence is proprietary) (Uniprot: P17947 )
Purification Method
Protein A or G purified
Research Discipline
Innate Immunity
Gene ID (Entrez)
6688.0
Target Species
Human
Form
Purified
Applications
Western Blot, Immunohistochemistry (Paraffin)
Clone
PU1/2146
Conjugate
Unconjugated
Formulation
10 mM PBS with No Preservative
Gene Alias
hematopoietic transcription factor PU.1, OF, PU.1, SFPI131 kDa transforming protein, SPI-1, SPI-1 proto-oncogene, SPI-A, spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein, transcription factor PU.1
Host Species
Mouse
Molecular Weight of Antigen
40 kDa
Quantity
100 μg
Primary or Secondary
Primary
Test Specificity
PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.
Content And Storage
Store at 4°C.
Isotype
IgG2b κ
Description
- Description PU.1/Spi-1 Monoclonal specifically detects PU.1/Spi-1 in Human samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Protein Array.
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Antigen: PU.1/Spi-1
Classification: Monoclonal
Concentration: 0.2 mg/ml
Dilution: Western Blot 0.5-1 ug/ml, Immunohistochemistry-Paraffin 1-2 ug/ml
Gene Accession No.: P17947
Gene Symbols: SPI1
Immunogen: Recombinant fragment (around aa 16-170) of human PU.1/Spi-1 protein (Exact sequence is proprietary) (Uniprot: P17947 )
Purification Method: Protein A or G purified
Research Discipline: Innate Immunity
Gene ID (Entrez): 6688.0
Target Species: Human
Form: Purified
Applications: Western Blot, Immunohistochemistry (Paraffin)
Clone: PU1/2146
Conjugate: Unconjugated
Formulation: 10 mM PBS with No Preservative
Gene Alias: hematopoietic transcription factor PU.1, OF, PU.1, SFPI131 kDa transforming protein, SPI-1, SPI-1 proto-oncogene, SPI-A, spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein, transcription factor PU.1
Host Species: Mouse
Molecular Weight of Antigen: 40 kDa
Quantity: 100 μg
Primary or Secondary: Primary
Test Specificity: PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.
Content And Storage: Store at 4°C.
Isotype: IgG2b κ
Antigen:
PU.1/Spi-1
Classification:
Monoclonal
Concentration:
0.2 mg/ml
Dilution:
Western Blot 0.5-1 ug/ml, Immunohistochemistry-Paraffin 1-2 ug/ml
Gene Accession No.:
P17947
Gene Symbols:
SPI1
Immunogen:
Recombinant fragment (around aa 16-170) of human PU.1/Spi-1 protein (Exact sequence is proprietary) (Uniprot: P17947 )
Purification Method:
Protein A or G purified
Research Discipline:
Innate Immunity
Gene ID (Entrez):
6688.0
Target Species:
Human
Form:
Purified
Applications:
Western Blot, Immunohistochemistry (Paraffin)
Clone:
PU1/2146
Conjugate:
Unconjugated
Formulation:
10 mM PBS with No Preservative
Gene Alias:
hematopoietic transcription factor PU.1, OF, PU.1, SFPI131 kDa transforming protein, SPI-1, SPI-1 proto-oncogene, SPI-A, spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein, transcription factor PU.1
Host Species:
Mouse
Molecular Weight of Antigen:
40 kDa
Quantity:
100 μg
Primary or Secondary:
Primary
Test Specificity:
PU.1 is a member of the ETS family of transcription factors and is important for normal B-cell development. It is expressed in the myeloid lineage and in immature as well as mature B-lymphocytes, with the exception of plasma cells. PU.1 is expressed in germinal center B-cells and mantle B-cells. Various lymphomas are also positive for this marker. It is essential during early B-cell differentiation. The absence of PU.1 results in total block of B-cell development at the pre-pro stage. PU.1 plays a key role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development.
Content And Storage:
Store at 4°C.
Isotype:
IgG2b κ
Antigen: Cytokeratin 1/10
Classification: Monoclonal
Concentration: 0.2 mg/ml
Dilution: Flow Cytometry 0.5-1 ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml, Immunohistochemistry-Paraffin 0.1-0.2 ug/ml
Gene Accession No.: P13645
Gene Symbols: KRT10
Immunogen: Recombinant human full-length Cytokeratin 10 protein (Uniprot: P13645)
Purification Method: Protein A or G purified
Research Discipline: Cytoskeleton Markers
Gene ID (Entrez): 3858.0
Target Species: Human, Mouse
Form: Purified
Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone: KRT10/1990R
Conjugate: Unconjugated
Formulation: 10 mM PBS with No Preservative
Gene Alias: 67 kDa cytokeratin, CK 1, CK 10, CK1, CK10, Cytokeratin 10, Cytokeratin1, Cytokeratin10, EHK 1, EHK1, EPPK, Hair Alpha Protein, K1, k10, Keratin 1, Keratin 1 (epidermolytic hyperkeratosis), Keratin 10, Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris), Keratin type I cytoskeletal 10, Keratin Type II Cytoskeletal 1, Keratin1, Keratin10, KPP, KRT 1, Krt 10, KRT1, KRT10, KRT1A, KRTA, NEPPK
Host Species: Rabbit
Molecular Weight of Antigen: 56.5 kDa
Quantity: 100 μg
Primary or Secondary: Primary
Test Specificity: This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Content And Storage: Store at 4°C.
Isotype: IgG
Antigen:
Cytokeratin 1/10
Classification:
Monoclonal
Concentration:
0.2 mg/ml
Dilution:
Flow Cytometry 0.5-1 ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1 ug/ml, Immunohistochemistry-Paraffin 0.1-0.2 ug/ml
Gene Accession No.:
P13645
Gene Symbols:
KRT10
Immunogen:
Recombinant human full-length Cytokeratin 10 protein (Uniprot: P13645)
Purification Method:
Protein A or G purified
Research Discipline:
Cytoskeleton Markers
Gene ID (Entrez):
3858.0
Target Species:
Human, Mouse
Form:
Purified
Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Clone:
KRT10/1990R
Conjugate:
Unconjugated
Formulation:
10 mM PBS with No Preservative
Gene Alias:
67 kDa cytokeratin, CK 1, CK 10, CK1, CK10, Cytokeratin 10, Cytokeratin1, Cytokeratin10, EHK 1, EHK1, EPPK, Hair Alpha Protein, K1, k10, Keratin 1, Keratin 1 (epidermolytic hyperkeratosis), Keratin 10, Keratin 10 (epidermolytic hyperkeratosis; keratosis palmaris et plantaris), Keratin type I cytoskeletal 10, Keratin Type II Cytoskeletal 1, Keratin1, Keratin10, KPP, KRT 1, Krt 10, KRT1, KRT10, KRT1A, KRTA, NEPPK
Host Species:
Rabbit
Molecular Weight of Antigen:
56.5 kDa
Quantity:
100 μg
Primary or Secondary:
Primary
Test Specificity:
This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Content And Storage:
Store at 4°C.
Isotype:
IgG
Antigen: Elastin alpha
Classification: Monoclonal
Concentration: 0.2 mg/ml
Dilution: ELISA, Immunohistochemistry-Paraffin 1-2 ug/ml
Gene Accession No.: P15502
Gene Symbols: ELN
Immunogen: Recombinant full-length human Elastin protein (Uniprot: P15502)
Purification Method: Protein A or G purified
Research Discipline: Extracellular Matrix
Gene ID (Entrez): 2006.0
Target Species: Human
Form: Purified
Applications: ELISA, Immunohistochemistry (Paraffin)
Clone: ELN/1981
Conjugate: Unconjugated
Formulation: 10 mM PBS with No Preservative
Gene Alias: elastin, FLJ38671, FLJ43523, SVAS, tropoelastin, WBS, WS
Host Species: Mouse
Molecular Weight of Antigen: 70 kDa
Quantity: 100 μg
Primary or Secondary: Primary
Test Specificity: Elastin is a polymeric protein and is the main component of the extracellular matrix of arteries. It is synthesized and secreted as a soluble, single-chain protein (tropoelastin), which undergoes a number of post-translational modifications prior to its organization into an elastic fiber in the extracellular space. Elastin performs a regulatory function during arterial development by controlling proliferation of smooth muscle and stabilizing arterial structure. It imparts elasticity to the connective tissue. During aging, the elasticity of connective tissue is reduced because of the cross-linking of collagenous fibers with elastin. The abnormal accumulation of elastic tissue in blood vessels is found in atherosclerosis and hypertension.
Content And Storage: Store at 4°C.
Isotype: IgG1 κ
Antigen:
Elastin alpha
Classification:
Monoclonal
Concentration:
0.2 mg/ml
Dilution:
ELISA, Immunohistochemistry-Paraffin 1-2 ug/ml
Gene Accession No.:
P15502
Gene Symbols:
ELN
Immunogen:
Recombinant full-length human Elastin protein (Uniprot: P15502)
Purification Method:
Protein A or G purified
Research Discipline:
Extracellular Matrix
Gene ID (Entrez):
2006.0
Target Species:
Human
Form:
Purified
Applications:
ELISA, Immunohistochemistry (Paraffin)
Clone:
ELN/1981
Conjugate:
Unconjugated
Formulation:
10 mM PBS with No Preservative
Gene Alias:
elastin, FLJ38671, FLJ43523, SVAS, tropoelastin, WBS, WS
Host Species:
Mouse
Molecular Weight of Antigen:
70 kDa
Quantity:
100 μg
Primary or Secondary:
Primary
Test Specificity:
Elastin is a polymeric protein and is the main component of the extracellular matrix of arteries. It is synthesized and secreted as a soluble, single-chain protein (tropoelastin), which undergoes a number of post-translational modifications prior to its organization into an elastic fiber in the extracellular space. Elastin performs a regulatory function during arterial development by controlling proliferation of smooth muscle and stabilizing arterial structure. It imparts elasticity to the connective tissue. During aging, the elasticity of connective tissue is reduced because of the cross-linking of collagenous fibers with elastin. The abnormal accumulation of elastic tissue in blood vessels is found in atherosclerosis and hypertension.
Content And Storage:
Store at 4°C.
Isotype:
IgG1 κ
Antigen: MyoD
Classification: Monoclonal
Concentration: 0.2 mg/ml
Dilution: Immunohistochemistry-Paraffin 0.5-1 ug/ml
Gene Accession No.: P15172, P15172
Gene Symbols: MYOD1
Immunogen: Recombinant human full-length MyoD protein (Uniprot: P15172 )
Purification Method: Protein A purified
Research Discipline: Cancer, Cell Cycle and Replication, Growth and Development, Phospho Specific
Gene ID (Entrez): 4654.0
Target Species: Human
Form: Purified
Applications: Immunohistochemistry (Paraffin)
Clone: MYOD1/2075R
Conjugate: Unconjugated
Formulation: 10 mM PBS with No Preservative
Gene Alias: bHLHc1BHLHC1, Class C basic helix-loop-helix protein 1, MYF3Myf-3, MYODmyoblast determination protein 1, myogenic differentiation 1, Myogenic factor 3myf-3, PUM
Host Species: Rabbit
Molecular Weight of Antigen: 45 kDa
Quantity: 100 μg
Primary or Secondary: Primary
Test Specificity: Recognizes a phospho-protein of 45kDa, identified as MyoD1. This monoclonal antibody does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Content And Storage: Store at 4°C.
Isotype: IgG
Antigen:
MyoD
Classification:
Monoclonal
Concentration:
0.2 mg/ml
Dilution:
Immunohistochemistry-Paraffin 0.5-1 ug/ml
Gene Accession No.:
P15172, P15172
Gene Symbols:
MYOD1
Immunogen:
Recombinant human full-length MyoD protein (Uniprot: P15172 )
Purification Method:
Protein A purified
Research Discipline:
Cancer, Cell Cycle and Replication, Growth and Development, Phospho Specific
Gene ID (Entrez):
4654.0
Target Species:
Human
Form:
Purified
Applications:
Immunohistochemistry (Paraffin)
Clone:
MYOD1/2075R
Conjugate:
Unconjugated
Formulation:
10 mM PBS with No Preservative
Gene Alias:
bHLHc1BHLHC1, Class C basic helix-loop-helix protein 1, MYF3Myf-3, MYODmyoblast determination protein 1, myogenic differentiation 1, Myogenic factor 3myf-3, PUM
Host Species:
Rabbit
Molecular Weight of Antigen:
45 kDa
Quantity:
100 μg
Primary or Secondary:
Primary
Test Specificity:
Recognizes a phospho-protein of 45kDa, identified as MyoD1. This monoclonal antibody does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas. Only nuclear staining should be considered as evidence of skeletal muscle differentiation.
Content And Storage:
Store at 4°C.
Isotype:
IgG