AM1200
Invitrogen™ Retic Lysate IVT™ Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1200-Each-of-1 | In Stock | ₹ 72,726.00 |
AM1200 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 72,726.00
GST (18%): ₹ 13,090.68
Total Price: ₹ 85,816.68
Content And Storage
• 5 × 200 μl (store at -80°C)• 2 × 1.75 ml Nuclease-free Water (store at any temperature)• 100 μl 20X Translation Mix minus-Met• 100 μl 20X Translation Mix minus-Leu• 100 μl High Salt Mix minus-Met• 100 μl Low Salt Mix minus-Met• 50 μl Capped Xef-1 RNA (50 μg/ml)Unless otherwise stated, store reagents below -70°C. Do not store cell-free translation systems in the liquid phase of a liquid nitrogen storage system.
Expressed Protein Type
Water Soluble Protein
Expression System
Cell-Free
No. of Reactions
60 Reactions
Product Type
Retic Lysate
Shipping Condition
Dry Ice
Format
Kit
Expression Mechanism
Cell-Free Expression
For Use With (Application)
In Vitro Transcription
Product Line
Ambion™
Quantity
60 Reactions
Related Products
Description
- The Retic Lysate IVT™ Kit efficiently translates in vitro-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs
- The optimized lysate results in high protein yield, biological activity, and consistent performance
- A report in Nucleic Acids Research (1) found that some commercial reticulocyte lysates had poor fidelity for initiation at the appropriate AUG initiator codon
- The Retic Lysate IVT Kit was tested using Kozak's constructs and it demonstrated high fidelity for initiating at the proper AUG
- Efficiently translates a wide variety of high molecular weight proteins and both capped and uncapped mRNAs Accommodates large volume of template mRNA How it works The lysate has been optimized for translation by the addition of an ATP-regenerating system, hemin, and calf liver tRNA
- Included in the kit is an optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers
- These buffers can be used to maximize protein yield and activity from difficult-to-translate sequences.
Compare Similar Items
Show Difference
Content And Storage: • 5 × 200 μl (store at -80°C)• 2 × 1.75 ml Nuclease-free Water (store at any temperature)• 100 μl 20X Translation Mix minus-Met• 100 μl 20X Translation Mix minus-Leu• 100 μl High Salt Mix minus-Met• 100 μl Low Salt Mix minus-Met• 50 μl Capped Xef-1 RNA (50 μg/ml)Unless otherwise stated, store reagents below -70°C. Do not store cell-free translation systems in the liquid phase of a liquid nitrogen storage system.
Expressed Protein Type: Water Soluble Protein
Expression System: Cell-Free
No. of Reactions: 60 Reactions
Product Type: Retic Lysate
Shipping Condition: Dry Ice
Format: Kit
Expression Mechanism: Cell-Free Expression
For Use With (Application): In Vitro Transcription
Product Line: Ambion™
Quantity: 60 Reactions
Content And Storage:
• 5 × 200 μl (store at -80°C)• 2 × 1.75 ml Nuclease-free Water (store at any temperature)• 100 μl 20X Translation Mix minus-Met• 100 μl 20X Translation Mix minus-Leu• 100 μl High Salt Mix minus-Met• 100 μl Low Salt Mix minus-Met• 50 μl Capped Xef-1 RNA (50 μg/ml)Unless otherwise stated, store reagents below -70°C. Do not store cell-free translation systems in the liquid phase of a liquid nitrogen storage system.
Expressed Protein Type:
Water Soluble Protein
Expression System:
Cell-Free
No. of Reactions:
60 Reactions
Product Type:
Retic Lysate
Shipping Condition:
Dry Ice
Format:
Kit
Expression Mechanism:
Cell-Free Expression
For Use With (Application):
In Vitro Transcription
Product Line:
Ambion™
Quantity:
60 Reactions
Content And Storage: Retic Lysate should be stored at –80°C. 20X Translation Mix minus-Met, 20X Translation Mix minus-Leu, High Salt Mix minus-Met, Low Salt Mix minus-Met, and Capped Xef-1 RNA should be stored below –70°C. Nuclease-Free water can be stored at any temperature.
Expressed Protein Type: Water Soluble Protein
Expression System: Cell-Free
No. of Reactions: 60 Reactions
Product Type: Retic Lysate
Shipping Condition: __
Format: Kit
Expression Mechanism: Cell-Free Expression
For Use With (Application): In Vitro Transcription
Product Line: Ambion™
Quantity: 60 Reactions
Content And Storage:
Retic Lysate should be stored at –80°C. 20X Translation Mix minus-Met, 20X Translation Mix minus-Leu, High Salt Mix minus-Met, Low Salt Mix minus-Met, and Capped Xef-1 RNA should be stored below –70°C. Nuclease-Free water can be stored at any temperature.
Expressed Protein Type:
Water Soluble Protein
Expression System:
Cell-Free
No. of Reactions:
60 Reactions
Product Type:
Retic Lysate
Shipping Condition:
__
Format:
Kit
Expression Mechanism:
Cell-Free Expression
For Use With (Application):
In Vitro Transcription
Product Line:
Ambion™
Quantity:
60 Reactions
Content And Storage: __
Expressed Protein Type: __
Expression System: __
No. of Reactions: __
Product Type: __
Shipping Condition: __
Format: __
Expression Mechanism: __
For Use With (Application): __
Product Line: __
Quantity: __
Content And Storage:
__
Expressed Protein Type:
__
Expression System:
__
No. of Reactions:
__
Product Type:
__
Shipping Condition:
__
Format:
__
Expression Mechanism:
__
For Use With (Application):
__
Product Line:
__
Quantity:
__
Content And Storage: __
Expressed Protein Type: __
Expression System: __
No. of Reactions: __
Product Type: __
Shipping Condition: __
Format: __
Expression Mechanism: __
For Use With (Application): __
Product Line: __
Quantity: __
Content And Storage:
__
Expressed Protein Type:
__
Expression System:
__
No. of Reactions:
__
Product Type:
__
Shipping Condition:
__
Format:
__
Expression Mechanism:
__
For Use With (Application):
__
Product Line:
__
Quantity:
__