AM1200M
Invitrogen™ Retic Lysate IVT™ Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1200M-Each-of-1 | In Stock | ₹ 73,581.60 |
AM1200M - Each of 1
In Stock
Quantity
1
Base Price: ₹ 73,581.60
GST (18%): ₹ 13,244.688
Total Price: ₹ 86,826.288
Content And Storage
Retic Lysate should be stored at –80°C. 20X Translation Mix minus-Met, 20X Translation Mix minus-Leu, High Salt Mix minus-Met, Low Salt Mix minus-Met, and Capped Xef-1 RNA should be stored below –70°C. Nuclease-Free water can be stored at any temperature.
Expressed Protein Type
Water Soluble Protein
Expression System
Cell-Free
No. of Reactions
60 Reactions
Product Type
Retic Lysate
Format
Kit
Expression Mechanism
Cell-Free Expression
For Use With (Application)
In Vitro Transcription
Product Line
Ambion™
Quantity
60 Reactions
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Description
- The Retic Lysate IVT™ Kit efficiently translates in vitro-synthesized transcripts, poly(A) RNA, and total RNA, including difficult-to-translate RNAs
- The optimized lysate results in high protein yield, biological activity, and consistent performance
- A report in Nucleic Acids Research (1) found that some commercial reticulocyte lysates had poor fidelity for initiation at the appropriate AUG initiator codon
- The Retic Lysate IVT Kit was tested using Kozak's constructs and it demonstrated high fidelity for initiating at the proper AUG
- Efficiently translates a wide variety of high molecular weight proteins and both capped and uncapped mRNAs Accommodates large volume of template mRNA How it works The lysate has been optimized for translation by the addition of an ATP-regenerating system, hemin, and calf liver tRNA
- Included in the kit is an optimized Translation Mix (-Met or -Leu) for the translation of both capped and uncapped messages along with High and Low Salt buffers
- These buffers can be used to maximize protein yield and activity from difficult-to-translate sequences.
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Content And Storage: Retic Lysate should be stored at –80°C. 20X Translation Mix minus-Met, 20X Translation Mix minus-Leu, High Salt Mix minus-Met, Low Salt Mix minus-Met, and Capped Xef-1 RNA should be stored below –70°C. Nuclease-Free water can be stored at any temperature.
Expressed Protein Type: Water Soluble Protein
Expression System: Cell-Free
No. of Reactions: 60 Reactions
Product Type: Retic Lysate
Format: Kit
Expression Mechanism: Cell-Free Expression
For Use With (Application): In Vitro Transcription
Product Line: Ambion™
Quantity: 60 Reactions
Content And Storage:
Retic Lysate should be stored at –80°C. 20X Translation Mix minus-Met, 20X Translation Mix minus-Leu, High Salt Mix minus-Met, Low Salt Mix minus-Met, and Capped Xef-1 RNA should be stored below –70°C. Nuclease-Free water can be stored at any temperature.
Expressed Protein Type:
Water Soluble Protein
Expression System:
Cell-Free
No. of Reactions:
60 Reactions
Product Type:
Retic Lysate
Format:
Kit
Expression Mechanism:
Cell-Free Expression
For Use With (Application):
In Vitro Transcription
Product Line:
Ambion™
Quantity:
60 Reactions
Content And Storage: __
Expressed Protein Type: __
Expression System: __
No. of Reactions: __
Product Type: __
Format: __
Expression Mechanism: __
For Use With (Application): __
Product Line: __
Quantity: __
Content And Storage:
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Expression System:
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No. of Reactions:
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Product Type:
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Format:
__
Expression Mechanism:
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For Use With (Application):
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Product Line:
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Quantity:
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Content And Storage: __
Expressed Protein Type: __
Expression System: __
No. of Reactions: __
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Format: __
Expression Mechanism: __
For Use With (Application): __
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Quantity: __
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Expression System:
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Content And Storage: Store at room temperature.
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Expression System: __
No. of Reactions: __
Product Type: RNase-free PCR Tube
Format: Tube
Expression Mechanism: __
For Use With (Application): __
Product Line: Ambion™
Quantity: 1000 tubes
Content And Storage:
Store at room temperature.
Expressed Protein Type:
__
Expression System:
__
No. of Reactions:
__
Product Type:
RNase-free PCR Tube
Format:
Tube
Expression Mechanism:
__
For Use With (Application):
__
Product Line:
Ambion™
Quantity:
1000 tubes