CD4 is an approximately 55kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T cells
In humans, CD4 is also expressed to a lesser extent on monocytes and macrophage related cells
Typical recoveries range between 5 and 35% and the purity of recovered cells ranges between 85 and 95% Kit protocols based on the use of Ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic) These Ferrofluids have a diameter of 150nm and as a result behave like colloidal particles, offering a higher ligand capacity per mass compared to other, larger magnetic particles