Interleukin-10 human

Description

• General description: Interleukin-10 gene with five exons is mapped to human chromosome 1. The gene codes for 35 kD protein containing 178 amino acids. IL-10 is a homodimer with two, non-covalently bonded monomers.[1]
• Application: Interleukin-10 human has been used to study its effect on activation of endogenous signal transducer and activator of transcription 1 (STAT1)/STAT3 in the knock-in, in A549 human lung adenocarcinoma cell line.[2]
• Biochem/physiol Actions: IL-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.
• Physical form: Lyophilized from a 0.2 μm filtered solution of 10mM Tris-HCl pH 7.4 + 150mM NaCl
• Preparation Note: HumanKine Interleukin-10 (IL-10) is expressed in human HEK 293 cells as a glycosylated, non-disulfide linked homodimer with an apparent molecular mass of 17 kDa due to glycosylation. Glycosylation contributes to stability in cell growth media and other applications.
• Analysis Note: The specific activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse master cell line) in presence of 200 pg/mL IL-4.
• Legal Information: HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc