RANK Ligand from mouse

Description

• General description: Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or receptor activator of nuclear factor-κB ligand (RANKL) is a type II transmembrane signaling receptor. It has a molecular weight of about 35kDa. The protein possesses an amino-terminal intracellular tail and a carboxy-terminal extracellular region, which has a connecting stalk and a receptor-binding domain.[1][2]
• Application: RANK ligand from mouse has been used to visualize differentiated osteoclasts and to stimulate osteoclast differentiation.[3]
• Biochem/physiol Actions: Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or RANKL has an important role in osteoclast function and differentiation. It binds to RANK and osteoprotegrin (OPG).[1][2] Bioactivities of RANKL may be attributed to its binding to these two receptor molecules. Mouse and human RANKL share 85% amino acid sequence identity.
• Physical form: Lyophilized from a 0.2 μm filtered solution of 5 mM sodium phosphate, pH 7.6, and 75 mM NaCl.
• Analysis Note: The bioactivity is determined by its ability to induce osteoclast differentiation of RAW 264.7. The expected ED50 for this effect is ≤10 ng/ml.