SRP6054
ProNGF human
recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
Manufacturer: Sigma Aldrich
CAS Number: 716844-28-9
Synonym(S): Beta-NGF, Beta-nerve growth factor
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 10 μG | SRP6054-10-μG | In Stock | ₹ 25,752.68 |
SRP6054 - 10 μG
In Stock
Quantity
1
Base Price: ₹ 25,752.68
GST (18%): ₹ 4,635.482
Total Price: ₹ 30,388.162
biological source
human
recombinant
expressed in E. coli
Assay
≥95% (HPLC)≥95% (SDS-PAGE)
form
lyophilized
mol wt
~25 kDa
packaging
pkg of 10 μg
NCBI accession no.
P01138
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... NGF(4803)
Description
- General description: The nerve growth factor (NGF) gene produces pro-nerve growth factor (proNGF), a soluble 246 amino acid pro-peptide.[1] NGF belongs to the neurotrophin family.[2] NGF gene is located on human chromosome 1p13.2.[3]
- Application: ProNGF human has been used as a standard in the de-glycosylation experiment for comparative studies by western blotting.[4]
- Biochem/physiol Actions: Pro-nerve growth factor (proNGF) participates in fetal neural development. It promotes neuronal survival and differentiation, or apoptosis, by interacting with particular nerve growth factor (NGF) receptors on neurons.[1] Haploinsufficiency of the nerve growth factor β (NGFB) gene results in sensory neuropathy.[3]
- Physical form: Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2.
- Preparation Note: Centrifuge the vial prior to opening.
- Reconstitution: Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/mL.). This can further be diluted to other aqueous buffers.
- Other Notes: Recombinant human ProNGF produced in E. coli is a non-glycosylated, non-covalently linked homodimer with each polypeptide chain containing 222 amino acids with an extra N-terminal Met and having a molecular mass of 25 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Pro-His-Ser.
SAFETY INFORMATION
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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biological source: human
recombinant: expressed in E. coli
Assay: ≥95% (HPLC)≥95% (SDS-PAGE)
form: lyophilized
mol wt: ~25 kDa
packaging: pkg of 10 μg
NCBI accession no.: P01138
shipped in: wet ice
storage temp.: −20°C
Gene Information: human ... NGF(4803)
biological source:
human
recombinant:
expressed in E. coli
Assay:
≥95% (HPLC)≥95% (SDS-PAGE)
form:
lyophilized
mol wt:
~25 kDa
packaging:
pkg of 10 μg
NCBI accession no.:
P01138
shipped in:
wet ice
storage temp.:
−20°C
Gene Information:
human ... NGF(4803)
biological source: human
recombinant: expressed in HEK 293 cells
Assay: ≥95% (SDS-PAGE)
form: lyophilized
mol wt: 36 kDa (monomer, glycosylated)
packaging: pkg of 10 μg
NCBI accession no.: P01588
shipped in: dry ice
storage temp.: __
Gene Information: __
biological source:
human
recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
form:
lyophilized
mol wt:
36 kDa (monomer, glycosylated)
packaging:
pkg of 10 μg
NCBI accession no.:
P01588
shipped in:
dry ice
storage temp.:
__
Gene Information:
__
biological source: human
recombinant: expressed in HEK 293 cells
Assay: ≥95% (SDS-PAGE)
form: lyophilized
mol wt: 22 kDa (monomer, non-glycosylated)
packaging: pkg of 10 μg
NCBI accession no.: P01241
shipped in: __
storage temp.: __
Gene Information: __
biological source:
human
recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
form:
lyophilized
mol wt:
22 kDa (monomer, non-glycosylated)
packaging:
pkg of 10 μg
NCBI accession no.:
P01241
shipped in:
__
storage temp.:
__
Gene Information:
__
biological source: human
recombinant: expressed in HEK 293 cells
Assay: ≥97% (SDS-PAGE)
form: lyophilized powder
mol wt: calculated mol wt 15 kDaobserved mol wt 18-23 kDa (DTT-reduced. Protein migrates due to glycosylation. Thr 103 is the predicted N-terminus .)
packaging: pkg of 10 μg
NCBI accession no.: __
shipped in: __
storage temp.: __
Gene Information: __
biological source:
human
recombinant:
expressed in HEK 293 cells
Assay:
≥97% (SDS-PAGE)
form:
lyophilized powder
mol wt:
calculated mol wt 15 kDaobserved mol wt 18-23 kDa (DTT-reduced. Protein migrates due to glycosylation. Thr 103 is the predicted N-terminus .)
packaging:
pkg of 10 μg
NCBI accession no.:
__
shipped in:
__
storage temp.:
__
Gene Information:
__