Description

• General description: 7α,25-dihydroxycholesterol (7α,25-OHC) is synthesized by the hydroxylation of cholesterol by the action of enzyme cholesterol 25-hydroxylase (CH25H) and cytochrome P450, family 7, subfamily b, polypeptide 1 (CYP7B1).[1] The catabolic breakdown of 7α,25-OHC to bile acid precursors is catalyzed by the enzymes hydroxy-Δ-5-steroid dehydrogenase, 3β- and steroid Δ-isomerase 7 (HSD3B7).[2]
• Application: 7α,25-dihydroxycholesterol has been used:as a G-protein-coupled receptor 183 ( GPR183) ligand in chemotaxis assay and intracellular cytokine staining method[2] and in fluorescence-activated cell sorting (FACS) analysis of natural killer cells[3] in oxysterol based calcium mobilization assay in Chinese hamster ovary (CHO) cells[1] in competitive radioligand binding assay of Epstein-Barr virus-induced gene 2 (EBI2) expressing COS-7 cells[4]
• Biochem/physiol Actions: 7α,25-dihydroxycholesterol (7α,25-OHC) serves as an endogenous ligand for G-protein-coupled receptor 183 (GPR183) or Epstein-Barr virus-induced gene 2 (EBI2). It plays a key role in the migration of type 3 innate lymphoid cells (ILC3) in the small intestine and colon. 7α,25-OHC mediates immune cell migration functionality by their chemoattractant property.[1] Inhibition of 7α,25-OHC synthesis leads to impairment in the migration of B cells.[4]
• Packaging: 5 mL Amber Glass Screw Cap Vial (700080P-1mg)