Description

• General description: 7-dehydrocholesterol (7-DHC) is a cholesterol precursor and is localized in basal epidermal keratinocytes.[1] 7DHC on photolysis in the presence of ultraviolet light B generates previtamin D3.[1] The enzyme 7-dehydrocholesterol reductase (DHCR7) catalyzes the reduction of C(7-8) double bond of 7-DHC to cholesterol.[2] 7-dehydrocholesterol-d7 is a deuterated derivative of 7-dehydrocholesterol.
• Application: 7-dehydrocholesterol-d7 has been used: as an internal standard: to spike Chlamydomonas CC-125 lipid extract for mass spectroscopy analysis[3]in liquid chromatography–mass spectrometry (LC-MS) for cultured cells oxysterol analysis[4]to treat and label SRD-1 cell (chinese hamster ovary (CHO) mutant cell-line) for gas chromatography/mass spectrometry (GC/MS) analysis[2]
• Biochem/physiol Actions: 7-Dehydrocholesterol (7-DHC) is used as a precursor for microbial vitamin D3 production. [5] Impairment of 7-dehydrocholesterol reductase (DHCR7) functionality leads to 7-DHC accumulation and is implicated in Smith-Lemli-Opitz syndrome (SLOS).[2]
• Packaging: 5 mL Amber Glass Screw Cap Vial (700116P-1mg)